OBJECTIVE: This study aimed to investigate the in vivo tissue performance of the association of Biosilicate(®) scaffolds and low-level laser therapy (LLLT) in a tibial bone defects model in rats. BACKGROUND DATA: Many studies have been demonstrating the osteogenic potential of Biosilicate and LLLT. However, there is a need to investigate the effects of both treatments for bone consolidation. METHODS: The animals were divided into control group (CG), Biosilicate scaffold group (BG), and Biosilicate scaffolds plus LLLT group (BLG). Animals were euthanized after 15, 30, and 45 days post-injury. RESULTS: The histological analysis revealed that all the experimental groups showed inflammatory infiltrate and granulation tissue, at the area of the defect at day 15. After 30 days, CG still showed granulation tissue and bone ingrowth. Both Biosilicate groups presented newly formed bone and interconected trabeculae. At 45 days, CG showed immature newly formed bone. A more mature newly formed bone was observed in BG and BLG. On day 15, BG demonstrated a statistically higher expression of cyclooxygenase (COX)-2 compared with CG and BLG. No statistically significant difference was observed in COX-2 immunoexpression among the groups at 30 and 45 days. Similar expression of bone morphogenetic protein (BMP)-9 was demonstrated for all experimental groups at 15 and 30 days. At 45 days, the BMP-9 immunoexpression was statistically upregulated in the BLG compared with the CG and BG. No statistically significant difference was observed in the receptor activator of nuclear factor kappa-B ligand (RANKL) immunoexpression among the groups in all periods evaluated. Biosilicate groups presented a decrease in biomechanical properties compared with CG at 30 and 45 days post-surgery. CONCLUSIONS: Our findings suggest that Biosilicate presented osteogenic activity, accelerating bone repair. However, laser therapy was not able to enhance the bioactive properties of the Biosilicate.
OBJECTIVE: This study aimed to investigate the in vivo tissue performance of the association of Biosilicate(®) scaffolds and low-level laser therapy (LLLT) in a tibial bone defects model in rats. BACKGROUND DATA: Many studies have been demonstrating the osteogenic potential of Biosilicate and LLLT. However, there is a need to investigate the effects of both treatments for bone consolidation. METHODS: The animals were divided into control group (CG), Biosilicate scaffold group (BG), and Biosilicate scaffolds plus LLLT group (BLG). Animals were euthanized after 15, 30, and 45 days post-injury. RESULTS: The histological analysis revealed that all the experimental groups showed inflammatory infiltrate and granulation tissue, at the area of the defect at day 15. After 30 days, CG still showed granulation tissue and bone ingrowth. Both Biosilicate groups presented newly formed bone and interconected trabeculae. At 45 days, CG showed immature newly formed bone. A more mature newly formed bone was observed in BG and BLG. On day 15, BG demonstrated a statistically higher expression of cyclooxygenase (COX)-2 compared with CG and BLG. No statistically significant difference was observed in COX-2 immunoexpression among the groups at 30 and 45 days. Similar expression of bone morphogenetic protein (BMP)-9 was demonstrated for all experimental groups at 15 and 30 days. At 45 days, the BMP-9 immunoexpression was statistically upregulated in the BLG compared with the CG and BG. No statistically significant difference was observed in the receptor activator of nuclear factor kappa-B ligand (RANKL) immunoexpression among the groups in all periods evaluated. Biosilicate groups presented a decrease in biomechanical properties compared with CG at 30 and 45 days post-surgery. CONCLUSIONS: Our findings suggest that Biosilicate presented osteogenic activity, accelerating bone repair. However, laser therapy was not able to enhance the bioactive properties of the Biosilicate.
Authors: Hueliton Wilian Kido; Carla Roberta Tim; Paulo Sérgio Bossini; Nivaldo Antônio Parizotto; Cynthia Aparecida de Castro; Murilo Camuri Crovace; Ana Candida Martins Rodrigues; Edgar Dutra Zanotto; Oscar Peitl Filho; Fernanda de Freitas Anibal; Ana Claudia Muniz Rennó Journal: J Mater Sci Mater Med Date: 2015-01-29 Impact factor: 3.896
Authors: Adrielle Martins Monteiro Alves; Lílian Melo de Miranda Fortaleza; Antonio Luiz Martins Maia Filho; Danniel Cabral Leão Ferreira; Charllyton Luis Sena da Costa; Vicente Galber Freitas Viana; José Zilton Lima Verde Santos; Rauirys Alencar de Oliveira; Gustavo Oliveira de Meira Gusmão; Luís Eduardo Silva Soares Journal: Lasers Med Sci Date: 2018-05-04 Impact factor: 3.161
Authors: P R Gabbai-Armelin; M T Souza; H W Kido; C R Tim; P S Bossini; A M P Magri; K R Fernandes; F A C Pastor; E D Zanotto; N A Parizotto; O Peitl; A C M Renno Journal: J Mater Sci Mater Med Date: 2015-04-17 Impact factor: 3.896
Authors: Angela Maria Paiva Magri; Kelly Rossetti Fernandes; Hueliton Wilian Kido; Gabriela Sodano Fernandes; Stephanie de Souza Fermino; Paulo Roberto Gabbai-Armelin; Franscisco José Correa Braga; Cíntia Pereirade Góes; José Lucas Dos Santos Prado; Renata Neves Granito; Ana Claudia Muniz Rennó Journal: J Mater Sci Mater Med Date: 2019-09-07 Impact factor: 3.896
Authors: Patricia Brassolatti; Paulo Sérgio Bossini; Ana Laura Martins de Andrade; Genoveva Lourdes Flores Luna; Juliana Virginio da Silva; Luciana Almeida-Lopes; Marcos Aurélio Napolitano; Lucimar Retto da Silva de Avó; Ângela Merice de Oliveira Leal; Fernanda de Freitas Anibal Journal: Acta Cir Bras Date: 2021-07-19 Impact factor: 1.388