INTRODUCTION: The need for studies that describe the resistance patterns in populations of Aedes aegypti (Linnaeus) in function of their region of origin justified this research, which aimed to characterize the resistance to temephos and to obtain information on esterase activity in populations of Aedes aegypti collected in municipalities of the State of Paraíba. METHODS: Resistance to temephos was evaluated and characterized from the diagnostic dose of 0.352mg i.a./L and multiple concentrations that caused mortalities between 5% and 99%. Electrophoresis of isoenzymes was used to verify the patterns of esterase activity among populations of the vector. RESULTS: All populations of Aedes aegypti were resistant to temephos, presenting a resistance rate (RR) greater than 20. The greatest lethal dose 50% of the sample (CL50) was found for the municipality of Lagoa Seca, approximately forty-one times the value of CL50 for the Rockefeller population. The populations characterized as resistant showed two to six regions of α and β-esterase, called EST-1 to EST-6, while the susceptible population was only seen in one region of activity. CONCLUSIONS: Aedes aegypti is widely distributed and shows a high degree of resistance to temephos in all municipalities studied. In all cases, esterases are involved in the metabolism and, consequently, in the resistance to temephos.
INTRODUCTION: The need for studies that describe the resistance patterns in populations of Aedes aegypti (Linnaeus) in function of their region of origin justified this research, which aimed to characterize the resistance to temephos and to obtain information on esterase activity in populations of Aedes aegypti collected in municipalities of the State of Paraíba. METHODS: Resistance to temephos was evaluated and characterized from the diagnostic dose of 0.352mg i.a./L and multiple concentrations that caused mortalities between 5% and 99%. Electrophoresis of isoenzymes was used to verify the patterns of esterase activity among populations of the vector. RESULTS: All populations of Aedes aegypti were resistant to temephos, presenting a resistance rate (RR) greater than 20. The greatest lethal dose 50% of the sample (CL50) was found for the municipality of Lagoa Seca, approximately forty-one times the value of CL50 for the Rockefeller population. The populations characterized as resistant showed two to six regions of α and β-esterase, called EST-1 to EST-6, while the susceptible population was only seen in one region of activity. CONCLUSIONS:Aedes aegypti is widely distributed and shows a high degree of resistance to temephos in all municipalities studied. In all cases, esterases are involved in the metabolism and, consequently, in the resistance to temephos.
Authors: Ana Paula de Araújo; Marcelo Henrique Santos Paiva; Amanda Maria Cabral; Antônio Emanuel Holanda Dias Cavalcanti; Luiz Fernando Freitas Pessoa; Diego Felipe Araujo Diniz; Elisama Helvecio; Ellyda Vanessa Gomes da Silva; Norma Machado da Silva; Daniela Bandeira Anastácio; Claudenice Pontes; Vânia Nunes; Maria de Fátima Marinho de Souza; Fernando Jorge Rodrigues Magalhães; Maria Alice Varjal de Melo Santos; Constância Flávia Junqueira Ayres Journal: J Insect Sci Date: 2019-05-01 Impact factor: 1.857
Authors: Mateus Chediak; Fabiano G Pimenta; Giovanini E Coelho; Ima A Braga; José Bento P Lima; Karina Ribeiro Lj Cavalcante; Lindemberg C de Sousa; Maria Alice V de Melo-Santos; Maria de Lourdes da G Macoris; Ana Paula de Araújo; Constância Flávia J Ayres; Maria Teresa M Andrighetti; Ricristhi Gonçalves de A Gomes; Kauara B Campos; Raul Narciso C Guedes Journal: Mem Inst Oswaldo Cruz Date: 2016-04-29 Impact factor: 2.743