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Literature DB >> 23733413

Photobleaching lifetimes of cyanine fluorophores used for single-molecule Förster resonance energy transfer in the presence of various photoprotection systems.

David Cooper¹, Heui Uhm, Lawrence J Tauzin, Nitesh Poddar, Christy F Landes.

Abstract

Lengthening smFRET lifetimes: We investigated various photoprotection system combinations to find the combination that optimally extended the photobleach lifetime of a Cy3/Cy5 smFRET pair attached to a DNA hairpin in a single-molecule environment. We found that the glucose/glucose oxygen-scavenging solution in combination with redox-based photostabilization solutions yielded the longest average photobleaching lifetimes.

Entities: Chemical Disease Gene Species

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Year: 2013 PMID： 23733413 PMCID： PMC3871170 DOI： 10.1002/cbic.201300030

Source DB: PubMed Journal: Chembiochem ISSN： 1439-4227 Impact factor: 3.164

39 in total

1. Information bounds and optimal analysis of dynamic single molecule measurements.

Authors: Lucas P Watkins; Haw Yang
Journal: Biophys J Date: 2004-06 Impact factor: 4.033

2. A photoprotection strategy for microsecond-resolution single-molecule fluorescence spectroscopy.

Authors: Luis A Campos; Jianwei Liu; Xiang Wang; Ravishankar Ramanathan; Douglas S English; Victor Muñoz
Journal: Nat Methods Date: 2011-01-09 Impact factor: 28.547

3. Nonblinking and long-lasting single-molecule fluorescence imaging.

Authors: Ivan Rasnik; Sean A McKinney; Taekjip Ha
Journal: Nat Methods Date: 2006-10-01 Impact factor: 28.547

4. Insights on the role of nucleic acid/protein interactions in chaperoned nucleic acid rearrangements of HIV-1 reverse transcription.

Authors: Hsiao-Wei Liu; Yining Zeng; Christy F Landes; Yoen Joo Kim; Yongjin Zhu; Xiaojing Ma; My-Nuong Vo; Karin Musier-Forsyth; Paul F Barbara
Journal: Proc Natl Acad Sci U S A Date: 2007-03-19 Impact factor: 11.205

5. Photophysics of backbone fluorescent DNA modifications: reducing uncertainties in FRET.

Authors: Suman Ranjit; Kaushik Gurunathan; Marcia Levitus
Journal: J Phys Chem B Date: 2009-06-04 Impact factor: 2.991

Review 6. A practical guide to single-molecule FRET.

Authors: Rahul Roy; Sungchul Hohng; Taekjip Ha
Journal: Nat Methods Date: 2008-06 Impact factor: 28.547

7. Improved photon yield from a green dye with a reducing and oxidizing system.

Authors: Antoine Le Gall; David Dulin; Gilles Clavier; Rachel Méallet-Renault; Philippe Bouyer; Karen Perronet; Nathalie Westbrook
Journal: Chemphyschem Date: 2011-05-31 Impact factor: 3.102

8. A triple threat to single molecules.

Authors: Martin Gruebele
Journal: Nat Methods Date: 2011-03 Impact factor: 28.547

9. Acidification of the oxygen scavenging system in single-molecule fluorescence studies: in situ sensing with a ratiometric dual-emission probe.

Authors: Xinghua Shi; John Lim; Taekjip Ha
Journal: Anal Chem Date: 2010-07-15 Impact factor: 6.986

6. Fluorescent labeling of plasmid DNA for gene delivery: Implications of dye hydrophobicity on labeling efficiencies and nanoparticle size.

Authors: Nathan A Delvaux; Basil Mathew; Kevin G Rice
Journal: Anal Biochem Date: 2020-08-09 Impact factor: 3.365

6 in total

Photobleaching lifetimes of cyanine fluorophores used for single-molecule Förster resonance energy transfer in the presence of various photoprotection systems.

1. Information bounds and optimal analysis of dynamic single molecule measurements.

2. A photoprotection strategy for microsecond-resolution single-molecule fluorescence spectroscopy.

3. Nonblinking and long-lasting single-molecule fluorescence imaging.

4. Insights on the role of nucleic acid/protein interactions in chaperoned nucleic acid rearrangements of HIV-1 reverse transcription.

5. Photophysics of backbone fluorescent DNA modifications: reducing uncertainties in FRET.

Review 6. A practical guide to single-molecule FRET.

7. Improved photon yield from a green dye with a reducing and oxidizing system.

8. A triple threat to single molecules.

9. Acidification of the oxygen scavenging system in single-molecule fluorescence studies: in situ sensing with a ratiometric dual-emission probe.

10. Structural landscape of isolated agonist-binding domains from single AMPA receptors.

1. Conformational transitions in the glycine-bound GluN1 NMDA receptor LBD via single-molecule FRET.

2. Engineering tandem single-chain Fv as cell surface reporters with enhanced properties of fluorescence detection.

3. Structural dynamics of the glycine-binding domain of the N-methyl-D-aspartate receptor.

4. Super-resolution mbPAINT for optical localization of single-stranded DNA.

Review 5. Molecular approaches to chromatography using single molecule spectroscopy.

6. Fluorescent labeling of plasmid DNA for gene delivery: Implications of dye hydrophobicity on labeling efficiencies and nanoparticle size.