Literature DB >> 21217750

A photoprotection strategy for microsecond-resolution single-molecule fluorescence spectroscopy.

Luis A Campos1, Jianwei Liu, Xiang Wang, Ravishankar Ramanathan, Douglas S English, Victor Muñoz.   

Abstract

Time resolution of current single-molecule fluorescence techniques is limited to milliseconds because of dye blinking and bleaching. Here we introduce a photoprotection strategy that affords microsecond resolution by combining efficient triplet quenching by oxygen and Trolox with minimized bleaching via the oxygen radical scavenger cysteamine. Using this approach we resolved the single-molecule microsecond conformational fluctuations of two proteins: the two-state folder α-spectrin SH3 domain and the ultrafast downhill folder BBL.

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Year:  2011        PMID： 21217750     DOI： 10.1038/nmeth.1553

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  15 in total

Review 1.  Fast kinetics and mechanisms in protein folding.

Authors:  W A Eaton; V Muñoz; S J Hagen; G S Jas; L J Lapidus; E R Henry; J Hofrichter
Journal:  Annu Rev Biophys Biomol Struct       Date:  2000

2.  Single-molecule spectroscopic determination of lac repressor-DNA loop conformation.

Authors:  Michael A Morgan; Kenji Okamoto; Jason D Kahn; Douglas S English
Journal:  Biophys J       Date:  2005-08-05       Impact factor: 4.033

3.  Analysis of single-molecule FRET trajectories using hidden Markov modeling.

Authors:  Sean A McKinney; Chirlmin Joo; Taekjip Ha
Journal:  Biophys J       Date:  2006-06-09       Impact factor: 4.033

4.  Photon counting histograms for diffusing fluorophores.

Authors:  Irina V Gopich; Attila Szabo
Journal:  J Phys Chem B       Date:  2005-09-22       Impact factor: 2.991

5.  Nonblinking and long-lasting single-molecule fluorescence imaging.

Authors:  Ivan Rasnik; Sean A McKinney; Taekjip Ha
Journal:  Nat Methods       Date:  2006-10-01       Impact factor: 28.547

Review 6.  Conformational dynamics and ensembles in protein folding.

Authors:  Victor Muñoz
Journal:  Annu Rev Biophys Biomol Struct       Date:  2007

7.  Single-molecule FRET measures bends and kinks in DNA.

Authors:  Anna K Wozniak; Gunnar F Schröder; Helmut Grubmüller; Claus A M Seidel; Filipp Oesterhelt
Journal:  Proc Natl Acad Sci U S A       Date:  2008-11-19       Impact factor: 11.205

Review 8.  A practical guide to single-molecule FRET.

Authors:  Rahul Roy; Sungchul Hohng; Taekjip Ha
Journal:  Nat Methods       Date:  2008-06       Impact factor: 28.547

9.  Mechanistic details of a protein-protein association pathway revealed by paramagnetic relaxation enhancement titration measurements.

Authors:  Nicolas L Fawzi; Michaeleen Doucleff; Jeong-Yong Suh; G Marius Clore
Journal:  Proc Natl Acad Sci U S A       Date:  2010-01-07       Impact factor: 11.205

10.  Direct observation of ultrafast folding and denatured state dynamics in single protein molecules.

Authors:  Hannes Neuweiler; Christopher M Johnson; Alan R Fersht
Journal:  Proc Natl Acad Sci U S A       Date:  2009-10-19       Impact factor: 11.205
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  37 in total

1.  Novel voltammetric tumor necrosis factor-alpha (TNF-α) immunosensor based on gold nanoparticles involved in thiol-functionalized multi-walled carbon nanotubes and bimetallic Ni/Cu-MOFs.

Authors:  Mehmet Lütfi Yola; Necip Atar
Journal:  Anal Bioanal Chem       Date:  2021-02-05       Impact factor: 4.142

Review 2.  Protein folding transition path times from single molecule FRET.

Authors:  Hoi Sung Chung; William A Eaton
Journal:  Curr Opin Struct Biol       Date:  2017-11-05       Impact factor: 6.809

Review 3.  The bright future of single-molecule fluorescence imaging.

Authors:  Manuel F Juette; Daniel S Terry; Michael R Wasserman; Zhou Zhou; Roger B Altman; Qinsi Zheng; Scott C Blanchard
Journal:  Curr Opin Chem Biol       Date:  2014-06-21       Impact factor: 8.822

Review 4.  Ultra-stable organic fluorophores for single-molecule research.

Authors:  Qinsi Zheng; Manuel F Juette; Steffen Jockusch; Michael R Wasserman; Zhou Zhou; Roger B Altman; Scott C Blanchard
Journal:  Chem Soc Rev       Date:  2014-02-21       Impact factor: 54.564

5.  A triple threat to single molecules.

Authors:  Martin Gruebele
Journal:  Nat Methods       Date:  2011-03       Impact factor: 28.547

6.  Evidence for the principle of minimal frustration in the evolution of protein folding landscapes.

Authors:  Franco O Tzul; Daniel Vasilchuk; George I Makhatadze
Journal:  Proc Natl Acad Sci U S A       Date:  2017-02-14       Impact factor: 11.205

7.  Variational Bayes analysis of a photon-based hidden Markov model for single-molecule FRET trajectories.

Authors:  Kenji Okamoto; Yasushi Sako
Journal:  Biophys J       Date:  2012-09-19       Impact factor: 4.033

8.  Four-color alternating-laser excitation single-molecule fluorescence spectroscopy for next-generation biodetection assays.

Authors:  Seok W Yim; Taiho Kim; Ted A Laurence; Steve Partono; Dongsik Kim; Younggyu Kim; Shimon Weiss; Armin Reitmair
Journal:  Clin Chem       Date:  2012-01-19       Impact factor: 8.327

9.  Conformations of a Metastable SH3 Domain Characterized by smFRET and an Excluded-Volume Polymer Model.

Authors:  Amir Mazouchi; Zhenfu Zhang; Abdullah Bahram; Gregory-Neal Gomes; Hong Lin; Jianhui Song; Hue Sun Chan; Julie D Forman-Kay; Claudiu C Gradinaru
Journal:  Biophys J       Date:  2016-04-12       Impact factor: 4.033

10.  Extending single molecule fluorescence observation time by amplitude-modulated excitation.

Authors:  Lydia Kisley; Wei-Shun Chang; David Cooper; Andrea P Mansur; Christy F Landes
Journal:  Methods Appl Fluoresc       Date:  2013-09-01       Impact factor: 3.009
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