Literature DB >> 23730004

Molecular Characterization of Phylogenetically Distinct Isolates of Grapevine fanleaf virus from Iran Based on 2A(HP) Gene.

Nemat Sokhandan-Bashir1, Azam Hooshmand, Afsaneh Delpasand-Khabazi.   

Abstract

Movement and coat protein genes from Grapevine fanleaf virus (GFLV) isolates have been characterized previously from Iran. In this study, an optimized reverse transcription polymerase chain reaction protocol was established to amplify RNA2 genomic segment corresponding to the hypothetical protein (2A(HP)). The sequence of 2A(HP) was compared with that of previously reported GFLV strains/isolates from other countries which showed 82-86% sequence identities. The 2A(HP) gene from Iran appeared to be standing distinct from other isolates of GFLV when genetic distance- or parsimony-based phylogeneitc analyses were carried out. The present study for the first time reports characterization of Iranian isolate of GFLV based on 2A(HP) gene.

Entities:  

Keywords:  2AHP gene; ELISA; GFLV; Hypothetical protein; Nepovirus; RT-PCR

Year:  2012        PMID： 23730004      PMCID： PMC3550808          DOI： 10.1007/s13337-012-0057-4

Source DB:  PubMed          Journal:  Indian J Virol        ISSN： 0970-2822


  18 in total

1.  Population structure and genetic variability within isolates of Grapevine fanleaf virus from a naturally infected vineyard in France: evidence for mixed infection and recombination.

Authors:  Emmanuelle Vigne; Marc Bergdoll; Sébastien Guyader; Marc Fuchs
Journal:  J Gen Virol       Date:  2004-08       Impact factor: 3.891

2.  Complete nucleotide sequences of the RNAs 2 of German isolates of grapevine fanleaf and Arabis mosaic nepoviruses.

Authors:  T Wetzel; L Meunier; U Jaeger; G M Reustle; G Krczal
Journal:  Virus Res       Date:  2001-06       Impact factor: 3.303

3.  Secoviridae: a proposed family of plant viruses within the order Picornavirales that combines the families Sequiviridae and Comoviridae, the unassigned genera Cheravirus and Sadwavirus, and the proposed genus Torradovirus.

Authors:  Hélène Sanfaçon; Joan Wellink; Olivier Le Gall; Alexander Karasev; René van der Vlugt; Thierry Wetzel
Journal:  Arch Virol       Date:  2009-04-07       Impact factor: 2.574

4.  Protein 2A of grapevine fanleaf nepovirus is implicated in RNA2 replication and colocalizes to the replication site.

Authors:  F Gaire; C Schmitt; C Stussi-Garaud; L Pinck; C Ritzenthaler
Journal:  Virology       Date:  1999-11-10       Impact factor: 3.616

5.  Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses.

Authors:  M F Clark; A N Adams
Journal:  J Gen Virol       Date:  1977-03       Impact factor: 3.891

6.  Genetic structure and molecular variability of Grapevine fanleaf virus populations.

Authors:  J E Oliver; E Vigne; M Fuchs
Journal:  Virus Res       Date:  2010-06-09       Impact factor: 3.303

7.  The nucleotide sequence of satellite RNA in grapevine fanleaf virus, strain F13.

Authors:  M Fuchs; M Pinck; M A Serghini; M Ravelonandro; B Walter; L Pinck
Journal:  J Gen Virol       Date:  1989-04       Impact factor: 3.891

8.  One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution.

Authors:  C T Chung; S L Niemela; R H Miller
Journal:  Proc Natl Acad Sci U S A       Date:  1989-04       Impact factor: 11.205

9.  RNA2 of grapevine fanleaf virus: sequence analysis and coat protein cistron location.

Authors:  M A Serghini; M Fuchs; M Pinck; J Reinbolt; B Walter; L Pinck
Journal:  J Gen Virol       Date:  1990-07       Impact factor: 3.891

10.  Cloning and in vitro characterization of the grapevine fanleaf virus proteinase cistron.

Authors:  R Margis; M Viry; M Pinck; L Pinck
Journal:  Virology       Date:  1991-12       Impact factor: 3.616
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