| Literature DB >> 23729249 |
Peter N Lowe1, Cara K Vaughan, Tina Daviter.
Abstract
Experimental approaches to detect, measure, and quantify protein-ligand binding, along with their theoretical bases, are described. A range of methods for detection of protein-ligand interactions is summarized. Specific protocols are provided for a nonequilibrium procedure pull-down assay, for an equilibrium direct binding method and its modification into a competition-based measurement and for steady-state measurements based on the effects of ligands on enzyme catalysis.Mesh：
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Year: 2013 PMID： 23729249 DOI： 10.1007/978-1-62703-398-5_3
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745