Literature DB >> 23728814

Engineering the meso-diaminopimelate dehydrogenase from Symbiobacterium thermophilum by site saturation mutagenesis for D-phenylalanine synthesis.

Xiuzhen Gao1, Fang Huang, Jinhui Feng, Xi Chen, Hailing Zhang, Zhixiang Wang, Qiaqing Wu, Dunming Zhu.   

Abstract

In order to enlarge the substrate binding pocket of the meso-diaminopimelate dehydrogenase from Symbiobacterium thermophilum to accommodate larger 2-keto acids, four amino acid residues (Phe146, Thr171, Arg181, and His227) were targeted for site saturation mutagenesis. Among all mutants, the single mutant H227V had a specific activity of 2.39 ± 0.06 U · mg(-1), which was 35.1-fold enhancement over the wild-type enzyme.

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Year:  2013        PMID： 23728814      PMCID： PMC3754726          DOI： 10.1128/AEM.01049-13

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  17 in total

1.  An efficient one-step site-directed and site-saturation mutagenesis protocol.

Authors:  Lei Zheng; Ulrich Baumann; Jean-Louis Reymond
Journal:  Nucleic Acids Res       Date:  2004-08-10       Impact factor: 16.971

2.  Predicting substrates by docking high-energy intermediates to enzyme structures.

Authors:  Johannes C Hermann; Eman Ghanem; Yingchun Li; Frank M Raushel; John J Irwin; Brian K Shoichet
Journal:  J Am Chem Soc       Date:  2006-12-13       Impact factor: 15.419

3.  Creation of a broad-range and highly stereoselective D-amino acid dehydrogenase for the one-step synthesis of D-amino acids.

Authors:  Kavitha Vedha-Peters; Manjula Gunawardana; J David Rozzell; Scott J Novick
Journal:  J Am Chem Soc       Date:  2006-08-23       Impact factor: 15.419

4.  Purification and properties of D-amino acid dehydrogenase, an inducible membrane-bound iron-sulfur flavoenzyme from Escherichia coli B.

Authors:  P J Olsiewski; G J Kaczorowski; C Walsh
Journal:  J Biol Chem       Date:  1980-05-25       Impact factor: 5.157

5.  A novel meso-Diaminopimelate dehydrogenase from Symbiobacterium thermophilum: overexpression, characterization, and potential for D-amino acid synthesis.

Authors:  Xiuzhen Gao; Xi Chen; Weidong Liu; Jinhui Feng; Qiaqing Wu; Ling Hua; Dunming Zhu
Journal:  Appl Environ Microbiol       Date:  2012-09-28       Impact factor: 4.792

6.  Redesign of the coenzyme specificity in L-lactate dehydrogenase from bacillus stearothermophilus using site-directed mutagenesis and media engineering.

Authors:  N Holmberg; U Ryde; L Bülow
Journal:  Protein Eng       Date:  1999-10

7.  Partial D-amino acid substitution: Improved enzymatic stability and preserved Ab recognition of a MUC2 epitope peptide.

Authors:  Regina Tugyi; Katalin Uray; Dóra Iván; Erzsébet Fellinger; Alan Perkins; Ferenc Hudecz
Journal:  Proc Natl Acad Sci U S A       Date:  2005-01-03       Impact factor: 11.205

8.  Substrate and inhibitor binding sites in Corynebacterium glutamicum diaminopimelate dehydrogenase.

Authors:  G Scapin; M Cirilli; S G Reddy; Y Gao; J C Vederas; J S Blanchard
Journal:  Biochemistry       Date:  1998-03-10       Impact factor: 3.162

9.  Meso-alpha,epsilon-diaminopimelate D-dehydrogenase: distribution and the reaction product.

Authors:  H Misono; H Togawa; T Yamamoto; K Soda
Journal:  J Bacteriol       Date:  1979-01       Impact factor: 3.490

10.  Biochemical profiling in silico--predicting substrate specificities of large enzyme families.

Authors:  Sadhna Tyagi; Juergen Pleiss
Journal:  J Biotechnol       Date:  2006-03-07       Impact factor: 3.307
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  9 in total

1.  A Newly Determined Member of the meso-Diaminopimelate Dehydrogenase Family with a Broad Substrate Spectrum.

Authors:  Xiuzhen Gao; Zheng Zhang; Ya'nan Zhang; Ying Li; Heng Zhu; Sheng Wang; Cun Li
Journal:  Appl Environ Microbiol       Date:  2017-05-17       Impact factor: 4.792

2.  Structure-Based Engineering of an Artificially Generated NADP+-Dependent d-Amino Acid Dehydrogenase.

Authors:  Junji Hayashi; Tomonari Seto; Hironaga Akita; Masahiro Watanabe; Tamotsu Hoshino; Kazunari Yoneda; Toshihisa Ohshima; Haruhiko Sakuraba
Journal:  Appl Environ Microbiol       Date:  2017-05-17       Impact factor: 4.792

3.  Efficient preparation of enantiopure D-phenylalanine through asymmetric resolution using immobilized phenylalanine ammonia-lyase from Rhodotorula glutinis JN-1 in a recirculating packed-bed reactor.

Authors:  Longbao Zhu; Li Zhou; Nan Huang; Wenjing Cui; Zhongmei Liu; Ke Xiao; Zhemin Zhou
Journal:  PLoS One       Date:  2014-09-30       Impact factor: 3.240

4.  Highly Atom Economic Synthesis of d-2-Aminobutyric Acid through an In Vitro Tri-enzymatic Catalytic System.

Authors:  Xi Chen; Yunfeng Cui; Xinkuan Cheng; Jinhui Feng; Qiaqing Wu; Dunming Zhu
Journal:  ChemistryOpen       Date:  2017-07-17       Impact factor: 2.911

Review 5.  Advances in Enzymatic Synthesis of D-Amino Acids.

Authors:  Loredano Pollegioni; Elena Rosini; Gianluca Molla
Journal:  Int J Mol Sci       Date:  2020-05-01       Impact factor: 5.923

6.  Highly selective synthesis of D-amino acids via stereoinversion of corresponding counterpart by an in vivo cascade cell factory.

Authors:  Dan-Ping Zhang; Xiao-Ran Jing; Lun-Jie Wu; An-Wen Fan; Yao Nie; Yan Xu
Journal:  Microb Cell Fact       Date:  2021-01-09       Impact factor: 5.328

7.  Highly selective synthesis of d-amino acids from readily available l-amino acids by a one-pot biocatalytic stereoinversion cascade.

Authors:  Danping Zhang; Xiaoran Jing; Wenli Zhang; Yao Nie; Yan Xu
Journal:  RSC Adv       Date:  2019-09-23       Impact factor: 4.036

8.  Improvement of biocatalysts for industrial and environmental purposes by saturation mutagenesis.

Authors:  Francesca Valetti; Gianfranco Gilardi
Journal:  Biomolecules       Date:  2013-10-08

9.  Supramolecular Chiral Discrimination of D-Phenylalanine Amino Acid Based on a Perylene Bisimide Derivative.

Authors:  Simona Bettini; Zois Syrgiannis; Michela Ottolini; Valentina Bonfrate; Gabriele Giancane; Ludovico Valli; Maurizio Prato
Journal:  Front Bioeng Biotechnol       Date:  2020-03-04
  9 in total

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