Literature DB >> 23728744

Isolation of Chlamydomonas flagella.

Branch Craige1, Jason M Brown, George B Witman.   

Abstract

A simple, scalable, and fast procedure for the isolation of Chlamydomonas flagella is described. Chlamydomonas can be synchronously deflagellated by treatment with chemicals, pH shock, or mechanical shear. The Basic Protocol describes the procedure for flagellar isolation using dibucaine to induce flagellar abscission; we also describe the pH shock method as an Alternate Protocol when flagellar regeneration is desirable. Sub-fractionation of the isolated flagella into axonemes and the membrane + matrix fraction is described in a Support Protocol.
© 2013 by John Wiley & Sons, Inc.

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Year:  2013        PMID： 23728744      PMCID： PMC3937870          DOI： 10.1002/0471143030.cb0341s59

Source DB:  PubMed          Journal:  Curr Protoc Cell Biol        ISSN： 1934-2616


  25 in total

1.  The chemical nature of the flagella of Chlamydomonas moewusii.

Authors:  R F JONES; R A LEWIN
Journal:  Exp Cell Res       Date:  1960-03       Impact factor: 3.905

2.  Purification and characterization of Chlamydomonas flagellar dyneins.

Authors:  S M King; T Otter; G B Witman
Journal:  Methods Enzymol       Date:  1986       Impact factor: 1.600

3.  Structural polarity and directional growth of microtubules of Chlamydomonas flagella.

Authors:  C Allen; G G Borisy
Journal:  J Mol Biol       Date:  1974-12-05       Impact factor: 5.469

4.  Identification of the flagellar proteins of Chlamydomonas reinhardii.

Authors:  M Jacobs; A McVittie
Journal:  Exp Cell Res       Date:  1970-11       Impact factor: 3.905

5.  Isolation of Chlamydomonas flagella and flagellar axonemes.

Authors:  G B Witman
Journal:  Methods Enzymol       Date:  1986       Impact factor: 1.600

6.  A FAP46 mutant provides new insights into the function and assembly of the C1d complex of the ciliary central apparatus.

Authors:  Jason M Brown; Christen G Dipetrillo; Elizabeth F Smith; George B Witman
Journal:  J Cell Sci       Date:  2012-05-08       Impact factor: 5.285

7.  Chlamydomonas flagellar mutants lacking radial spokes and central tubules. Structure, composition, and function of specific axonemal components.

Authors:  G B Witman; J Plummer; G Sander
Journal:  J Cell Biol       Date:  1978-03       Impact factor: 10.539

8.  Nonlethal deciliation of Tetrahymena by a local anesthetic and its utility as a tool for studying cilia regeneration.

Authors:  G A Thompson; L C Baugh; L F Walker
Journal:  J Cell Biol       Date:  1974-04       Impact factor: 10.539

9.  Localization of calmodulin and dynein light chain LC8 in flagellar radial spokes.

Authors:  P Yang; D R Diener; J L Rosenbaum; W S Sale
Journal:  J Cell Biol       Date:  2001-06-11       Impact factor: 10.539

10.  Chlamydomonas flagella. I. Isolation and electrophoretic analysis of microtubules, matrix, membranes, and mastigonemes.

Authors:  G B Witman; K Carlson; J Berliner; J L Rosenbaum
Journal:  J Cell Biol       Date:  1972-09       Impact factor: 10.539
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  11 in total

1.  How Does Cilium Length Affect Beating?

Authors:  Mathieu Bottier; Kyle A Thomas; Susan K Dutcher; Philip V Bayly
Journal:  Biophys J       Date:  2019-02-26       Impact factor: 4.033

2.  Assembly of IFT trains at the ciliary base depends on IFT74.

Authors:  Jason M Brown; Deborah A Cochran; Branch Craige; Tomohiro Kubo; George B Witman
Journal:  Curr Biol       Date:  2015-06-04       Impact factor: 10.834

3.  Ciliogenesis requires sphingolipid-dependent membrane and axoneme interaction.

Authors:  Dou Wu; Jingying Huang; Hao Zhu; Zhe Chen; Yongping Chai; Jingyi Ke; Kexin Lei; Zhao Peng; Ranhao Zhang; Xueming Li; Kaiyao Huang; Wei Li; Chengtian Zhao; Guangshuo Ou
Journal:  Proc Natl Acad Sci U S A       Date:  2022-07-27       Impact factor: 12.779

4.  Developmental changes in ciliary composition during gametogenesis in Chlamydomonas.

Authors:  Miho Sakato-Antoku; Stephen M King
Journal:  Mol Biol Cell       Date:  2022-04-07       Impact factor: 3.612

5.  Electron microscopy for imaging organelles in plants and algae.

Authors:  Ethan Weiner; Justine M Pinskey; Daniela Nicastro; Marisa S Otegui
Journal:  Plant Physiol       Date:  2022-02-04       Impact factor: 8.005

6.  Characterization of the truncated hemoglobin THB1 from protein extracts of Chlamydomonas reinhardtii.

Authors:  Eric A Johnson; Juliette T J Lecomte
Journal:  F1000Res       Date:  2014-12-04

7.  Chlamydomonas FAP265 is a tubulin polymerization promoting protein, essential for flagellar reassembly and hatching of daughter cells from the sporangium.

Authors:  Damayanti Tammana; Trinadh Venkata Satish Tammana
Journal:  PLoS One       Date:  2017-09-20       Impact factor: 3.240

8.  IFT54 directly interacts with kinesin-II and IFT dynein to regulate anterograde intraflagellar transport.

Authors:  Xin Zhu; Jieling Wang; Shufen Li; Karl Lechtreck; Junmin Pan
Journal:  EMBO J       Date:  2020-12-28       Impact factor: 11.598

9.  A bioactive peptide amidating enzyme is required for ciliogenesis.

Authors:  Dhivya Kumar; Daniela Strenkert; Ramila S Patel-King; Michael T Leonard; Sabeeha S Merchant; Richard E Mains; Stephen M King; Betty A Eipper
Journal:  Elife       Date:  2017-05-17       Impact factor: 8.140

10.  Mutations in PIH proteins MOT48, TWI1 and PF13 define common and unique steps for preassembly of each, different ciliary dynein.

Authors:  Ryosuke Yamamoto; Shiho Yanagi; Masahito Nagao; Yuya Yamasaki; Yui Tanaka; Winfield S Sale; Toshiki Yagi; Takahide Kon
Journal:  PLoS Genet       Date:  2020-11-03       Impact factor: 5.917
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