| Literature DB >> 23721661 |
Markênia Kélia Santos Alves1, Mário Henrique Girão Faria, Eduardo Henrique Cunha Neves Filho, Adriana Camargo Ferrasi, Maria Inês de Moura Campos Pardini, Manoel Odorico de Moraes Filho, Silvia Helena Barem Rabenhorst.
Abstract
CDKN2A promoter hypermethylation has been widely related to many cancers. In astrocytomas, although CDKN2A (p16(INK4A) protein) is often inactivated, there are still some controversial issues regarding the mechanism by which this alteration occurs. Thus, we analyzed a series of astrocytomas to assess the association between CDKN2A expression and methylation of grade I-IV tumors (WHO) and clinicopathological parameters. DNA extracted from formalin-fixed paraffin-embedded material of 93 astrocytic tumors was available for CDKN2A promoter methylation analysis and p16(INK4A) expression by methylation-specific PCR and immunohistochemistry, respectively. A strong negative correlation between nuclear and cytoplasmic immunostaining and CDKN2A promoter methylation was found. Additionally, a significant negative correlation between CDKN2A promoter methylation and age was observed; also, female patients had statistically more CDKN2A methylated promoters (p = 0.036) than men. In conclusion, CDKN2A inactivation by promoter methylation is a frequent event in astrocytomas and it is related to the age and sex of patients.Entities:
Keywords: Age; Astrocytic tumors; CDKN2A Methylation; Expression; p16
Mesh：
Substances：
Year: 2013 PMID： 23721661 DOI： 10.1016/j.ijsu.2013.05.030
Source DB: PubMed Journal: Int J Surg ISSN： 1743-9159 Impact factor: 6.071