BACKGROUND: Cockroaches produce potent allergens, and cockroach feces are known to be especially rich in allergens. In this study, we analyze the allergenic components from cockroach feces and evaluate allergenicity of recombinant α-amylase identified from fecal extract. METHODS: IgE-reactive proteins from German cockroach fecal extract were analyzed by proteomic analysis and immunoblotting. Recombinant α-amylase was produced and its allergenicity was evaluated by ELISA. RESULTS: Analysis of German cockroach fecal extracts identified 12 IgE-reactive components. Most of these allergens were found to be digestive enzymes such as α-amylase, trypsin, chymotrypsin, metalloprotease, and midgut carboxypeptidase A, but the identity of 3 IgE-reactive proteins is still unknown. Glycinin-like proteins, which were likely derived from the cockroach diet, were also identified. German cockroach α-amylase shares the highest identity with pig α-amylase (55.8%), followed by mite group 4 allergens (Blo t 4, 50.4%; Der p 4, 49.8%; Eur m 4, 47.4%). In this study, recombinant α-amylase from German cockroach was expressed, and its allergenicity was examined by ELISA. Specific IgE against recombinant amylase was detected in 41.4% (12/29) of serum samples from German cockroach-sensitized subjects. Recombinant α-amylase was able to inhibit 55% of specific IgE to German cockroach whole-body extract. CONCLUSIONS: Amylase was found to be an important novel allergen in cockroach feces. It is hoped that recombinant α-amylase will be useful for further studies and clinical applications.
BACKGROUND: Cockroaches produce potent allergens, and cockroach feces are known to be especially rich in allergens. In this study, we analyze the allergenic components from cockroach feces and evaluate allergenicity of recombinant α-amylase identified from fecal extract. METHODS: IgE-reactive proteins from German cockroach fecal extract were analyzed by proteomic analysis and immunoblotting. Recombinant α-amylase was produced and its allergenicity was evaluated by ELISA. RESULTS: Analysis of German cockroach fecal extracts identified 12 IgE-reactive components. Most of these allergens were found to be digestive enzymes such as α-amylase, trypsin, chymotrypsin, metalloprotease, and midgut carboxypeptidase A, but the identity of 3 IgE-reactive proteins is still unknown. Glycinin-like proteins, which were likely derived from the cockroach diet, were also identified. German cockroach α-amylase shares the highest identity with pig α-amylase (55.8%), followed by mite group 4 allergens (Blo t 4, 50.4%; Der p 4, 49.8%; Eur m 4, 47.4%). In this study, recombinant α-amylase from German cockroach was expressed, and its allergenicity was examined by ELISA. Specific IgE against recombinant amylase was detected in 41.4% (12/29) of serum samples from German cockroach-sensitized subjects. Recombinant α-amylase was able to inhibit 55% of specific IgE to German cockroach whole-body extract. CONCLUSIONS: Amylase was found to be an important novel allergen in cockroach feces. It is hoped that recombinant α-amylase will be useful for further studies and clinical applications.
Authors: L Karla Arruda; Michelle C R Barbosa; Ana Beatriz R Santos; Adriana S Moreno; Martin D Chapman; Anna Pomés Journal: Curr Allergy Asthma Rep Date: 2014-04 Impact factor: 4.806
Authors: Anna Pomés; Geoffrey A Mueller; Thomas A Randall; Martin D Chapman; L Karla Arruda Journal: Curr Allergy Asthma Rep Date: 2017-04 Impact factor: 4.806
Authors: Anna Pomés; Jill Glesner; Agustin Calatroni; Cindy M Visness; Robert A Wood; George T O'Connor; Meyer Kattan; Leonard B Bacharier; Lisa M Wheatley; James E Gern; William W Busse Journal: J Allergy Clin Immunol Date: 2019-06-13 Impact factor: 10.793
Authors: Jill Glesner; Stephanie Filep; Lisa D Vailes; Sabina Wünschmann; Martin D Chapman; Giovanni Birrueta; April Frazier; Kyoung Yong Jeong; Coby Schal; Leonard Bacharier; Avraham Beigelman; Paula Busse; Véronique Schulten; Alessandro Sette; Anna Pomés Journal: J Allergy Clin Immunol Date: 2018-08-28 Impact factor: 10.793
Authors: M B C Dillon; V Schulten; C Oseroff; S Paul; L M Dullanty; A Frazier; X Belles; M D Piulachs; C Visness; L Bacharier; G R Bloomberg; P Busse; J Sidney; B Peters; A Sette Journal: Clin Exp Allergy Date: 2015-12 Impact factor: 5.018
Authors: Kyoung Yong Jeong; Mina Son; Jae Hyun Lee; Chein Soo Hong; Jung Won Park Journal: Allergy Asthma Immunol Res Date: 2014-12-18 Impact factor: 5.764