| Literature DB >> 23684926 |
Andrea Canitano1, Giulietta Venturi, Martina Borghi, Maria Grazia Ammendolia, Stefano Fais.
Abstract
EBV is a human herpesvirus associated with a number of malignancies. Both lymphoblastoid cell lines (LCLs), and EBV-infected nasopharyngeal carcinoma (NPC) cells have been demonstrated to release exosomes containing the EBV-encoded latent membrane protein 1 (LMP1), and mature micro-RNAs (EBV-miRNAs). Here we analyze the EBV protein and nucleic acid content of exosomes from different EBV-infected cells (LCL, 721 and Daudi) and we show for the first time that exosomes released from LCLs and 721 also contain EBV-encoded latent phase mRNAs. This confirms and strengthens exosomes pathogenetic potential, and might provide insights for development of novel diagnostic and therapeutic strategies.Entities:
Keywords: EBV; Epstein–Barr virus; Exosomes; LCL; LMP; Latent infection; RT; Real-Time PCR; latent membrane protein; lymphoblastoid cell line; mRNA; messanger RNA; miRNA; micro RNA; qPCR; reverse transcription
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Year: 2013 PMID： 23684926 DOI： 10.1016/j.canlet.2013.05.012
Source DB: PubMed Journal: Cancer Lett ISSN： 0304-3835 Impact factor: 8.679