OBJECTIVE: To analyse the contribution of the Ogawa-Kudoh (O-K) swab culture method to the diagnosis of pulmonary tuberculosis (PTB) in four different regions of Brazil. DESIGN: This study was carried out in two phases. Phase 1 was designed to compare the direct swab culture method (O-K) with the culture concentrated method (N-acetyl-L-cysteine-sodium hydroxide [NALC-NaOH]); for this purpose, 569 sputum samples were cultured by both methods. Phase 2 was carried out to assess the contribution of the O-K method to the diagnosis of PTB in four different regions in Brazil, based on the evaluation of 19,163 sputum samples. RESULTS: In the first phase of the study, O-K culture had a sensitivity of 94.8% and specificity of 99.8% in cases confirmed by NALC-NaOH/Löwenstein-Jensen (LJ) culture. In the second phase of the study, the overall contribution of O-K culture compared to acid-fast bacilli (AFB) examination (AFB-/culture+) to the diagnosis of PTB was 29.8%. CONCLUSION: O-K culture contributes significantly to the diagnosis of smear-negative PTB. Importantly, this method allows the recovery of clinical isolates in areas where use of the standard culture centrifuge is impossible, indicating that the O-K swab culture method should become a standard method for TB diagnosis in these regions.
OBJECTIVE: To analyse the contribution of the Ogawa-Kudoh (O-K) swab culture method to the diagnosis of pulmonary tuberculosis (PTB) in four different regions of Brazil. DESIGN: This study was carried out in two phases. Phase 1 was designed to compare the direct swab culture method (O-K) with the culture concentrated method (N-acetyl-L-cysteine-sodium hydroxide [NALC-NaOH]); for this purpose, 569 sputum samples were cultured by both methods. Phase 2 was carried out to assess the contribution of the O-K method to the diagnosis of PTB in four different regions in Brazil, based on the evaluation of 19,163 sputum samples. RESULTS: In the first phase of the study, O-K culture had a sensitivity of 94.8% and specificity of 99.8% in cases confirmed by NALC-NaOH/Löwenstein-Jensen (LJ) culture. In the second phase of the study, the overall contribution of O-K culture compared to acid-fast bacilli (AFB) examination (AFB-/culture+) to the diagnosis of PTB was 29.8%. CONCLUSION:O-K culture contributes significantly to the diagnosis of smear-negative PTB. Importantly, this method allows the recovery of clinical isolates in areas where use of the standard culture centrifuge is impossible, indicating that the O-K swab culture method should become a standard method for TB diagnosis in these regions.
Authors: Greta Franco-Sotomayor; Ismar A Rivera-Olivero; Margarita Leon-Benitez; Sandra E Uruchima-Campoverde; Greta Cardenas-Franco; Mercedes E Perdomo-Castro; Camilo S Cardenas-Franco; Jackeline Ortega-Vivanco; Ana S Abad-Ruiz; Jacobus H de Waard; Miguel Angel Garcia-Bereguiain Journal: J Clin Microbiol Date: 2020-03-25 Impact factor: 5.948
Authors: Ronaldo Rodrigues da Costa; Marcio Roberto Silva; Claudio Jose Augusto; Isabel Cristina Gonçalves Leite Journal: Trans R Soc Trop Med Hyg Date: 2022-06-01 Impact factor: 2.455
Authors: Eunice Atsuko Cunha; Lucilaine Ferrazoli; Lee W Riley; Paulo Cesar Basta; Michael Robert Honer; Rosalia Maia; Izaias Pereira da Costa Journal: Mem Inst Oswaldo Cruz Date: 2013-11-04 Impact factor: 2.743
Authors: Carlos Acuña-Villaorduña; Luiz Guilherme Schmidt-Castellani; Patricia Marques-Rodrigues; Laura F White; David Jamil Hadad; Mary Gaeddert; Jerrold J Ellner; Kevin P Fennelly; Moises Palaci; Reynaldo Dietze; Edward C Jones-López Journal: PLoS One Date: 2018-10-29 Impact factor: 3.240