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Literature DB >> 23674216

Protocol: ex vivo culture of mouse embryonic mammary buds.

Maria Voutilainen¹, Päivi H Lindfors, Marja L Mikkola.

Abstract

The explant culture techniques of embryonic tissues allow continuous monitoring of organ growth and morphogenesis ex vivo. The effect of growth factors and other soluble molecules can be examined by applying them to the culture medium. Relatively few studies have reported application of tissue culture techniques to analysis of embryonic mammary glands. Here we describe a protocol for murine mammary rudiments that permits ex vivo development up to branching stage.

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Year: 2013 PMID： 23674216 DOI： 10.1007/s10911-013-9288-2

Source DB: PubMed Journal: J Mammary Gland Biol Neoplasia ISSN： 1083-3021 Impact factor: 2.673

13 in total

1. Explant cultures of embryonic epithelium. Analysis of mesenchymal signals.

Authors: Carin Sahlberg; Tuija Mustonen; Irma Thesleff
Journal: Methods Mol Biol Date: 2002

2. Explant culture of embryonic craniofacial tissues: analyzing effects of signaling molecules on gene expression.

Authors: Katja Närhi; Irma Thesleff
Journal: Methods Mol Biol Date: 2010

3. In vitro analysis of the hormonal basis for the sexual dimorphism in the embryonic development of the mouse mammary gland.

Authors: K Kratochwil
Journal: J Embryol Exp Morphol Date: 1971-02

4. Regression of mouse mammary gland anlagen in recombinants of Tfm and wild-type tissues: testosterone acts via the mesenchyme.

Authors: U Drews; U Drews
Journal: Cell Date: 1977-03 Impact factor: 41.582

5. Development and loss of androgen responsiveness in the embryonic rudiment of the mouse mammary gland.

Authors: K Kratochwil
Journal: Dev Biol Date: 1977-12 Impact factor: 3.582

6. Ectodysplasin regulates hormone-independent mammary ductal morphogenesis via NF-κB.

Authors: Maria Voutilainen; Päivi H Lindfors; Sylvie Lefebvre; Laura Ahtiainen; Ingrid Fliniaux; Elisa Rysti; Marja Murtoniemi; Pascal Schneider; Ruth Schmidt-Ullrich; Marja L Mikkola
Journal: Proc Natl Acad Sci U S A Date: 2012-03-26 Impact factor: 11.205

7. BMP4 and PTHrP interact to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction.

Authors: Julie R Hens; Pamela Dann; Jian-Ping Zhang; Stephen Harris; Gertraud W Robinson; John Wysolmerski
Journal: Development Date: 2007-02-14 Impact factor: 6.868

Review 8. Mouse embryonic mammogenesis as a model for the molecular regulation of pattern formation.

Authors: Jacqueline M Veltmaat; Arnaud A Mailleux; Jean Paul Thiery; Saverio Bellusci
Journal: Differentiation Date: 2003-01 Impact factor: 3.880

9. Embryonic mammary anlagen analysis using immunolabelling of whole mounts.

Authors: Heena Panchal; Olivia Wansbury; Beatrice A Howard
Journal: Methods Mol Biol Date: 2010

Review 10. Prenatal morphogenesis of mammary glands in mouse and rabbit.

Authors: Alain Y Propper; Beatrice A Howard; Jacqueline M Veltmaat
Journal: J Mammary Gland Biol Neoplasia Date: 2013-06-05 Impact factor: 2.673

7 in total

1. Multidimensional Fluorescence Imaging of Embryonic and Postnatal Mammary Gland Development.

Authors: Claudia Carabaña; Bethan Lloyd-Lewis
Journal: Methods Mol Biol Date: 2022

2. Protocol for Studying Embryonic Mammary Gland Branching Morphogenesis Ex Vivo.

Authors: Qiang Lan; Jyoti Satta; Satu-Marja Myllymäki; Ewelina Trela; Riitta Lindström; Beata Kaczyńska; Johanna Englund; Marja L Mikkola
Journal: Methods Mol Biol Date: 2022

3. New insights into fetal mammary gland morphogenesis: differential effects of natural and environmental estrogens.

Authors: Lucia Speroni; Maria Voutilainen; Marja L Mikkola; Skylar A Klager; Cheryl M Schaeberle; Carlos Sonnenschein; Ana M Soto
Journal: Sci Rep Date: 2017-01-19 Impact factor: 4.379

Review 4. Investigating molecular mechanisms of embryonic mammary gland development by bead-implantation in embryonic flank explant cultures - a protocol.

Authors: Jacqueline M Veltmaat
Journal: J Mammary Gland Biol Neoplasia Date: 2013-05-26 Impact factor: 2.673