Explant culture of embryonic craniofacial tissues: analyzing effects of signaling molecules on gene expression.

The in vitro culture of embryonic tissue explants allows the continuous monitoring of growth and morphogenesis at specific embryonic stages. The functions of soluble regulatory molecules can be examined by adding them into culture medium or by introducing them with beads to specific locations in the tissue. Gene expression analysis using in situ hybridization, quantitative PCR, and reporter constructs can be combined with organ culture to examine the functions of the regulatory molecules.