Jie Lu1, Xiaomei Feng, Yuqian Han, Changhu Xue. 1. Department of Food Science and Engineering, Ocean University of China, Qingdao, 266003, People's Republic of China.
Abstract
BACKGROUND: The carotenoids and chlorophyll a of Laminaria japonica Aresch were extracted using ethanol-modified subcritical 1,1,1,2-tetrafluoroethane (R134a). In the present study, the effects of pressure (5-17 MPa), temperature (303-333 K) and the amount of cosolvent(2-6% R134a, w/w)were investigated. Response surface methodology (RSM) combined with a Box-Behnken design was applied to evaluate the significance of the three independent variables on each response. A desirability function was conducted to simultaneously optimize the multiple responses. RESULTS: The optimum extraction conditions were as follows: extraction temperature 324.13 K, extraction pressure 17 MPa and a cosolvent amount of 4.73%. Under these conditions, the yields of carotenoids and chlorophyll a were predicted to be 0.239 and 2.326 g kg(-1), respectively. CONCLUSION: It has been proved that subcritical R134a is a potential solvent, which can be an alternative to supercritical CO2 for extraction of natural ingredients under mild conditions.
BACKGROUND: The carotenoids and chlorophyll a of Laminaria japonica Aresch were extracted using ethanol-modified subcritical 1,1,1,2-tetrafluoroethane (R134a). In the present study, the effects of pressure (5-17 MPa), temperature (303-333 K) and the amount of cosolvent(2-6% R134a, w/w)were investigated. Response surface methodology (RSM) combined with a Box-Behnken design was applied to evaluate the significance of the three independent variables on each response. A desirability function was conducted to simultaneously optimize the multiple responses. RESULTS: The optimum extraction conditions were as follows: extraction temperature 324.13 K, extraction pressure 17 MPa and a cosolvent amount of 4.73%. Under these conditions, the yields of carotenoids and chlorophyll a were predicted to be 0.239 and 2.326 g kg(-1), respectively. CONCLUSION: It has been proved that subcritical R134a is a potential solvent, which can be an alternative to supercritical CO2 for extraction of natural ingredients under mild conditions.
Authors: Mahesha M Poojary; Francisco J Barba; Bahar Aliakbarian; Francesco Donsì; Gianpiero Pataro; Daniel A Dias; Pablo Juliano Journal: Mar Drugs Date: 2016-11-22 Impact factor: 5.118