| Literature DB >> 23666702 |
Lawrence M Weiss1, Yuan-Yuan Chen.
Abstract
Epstein-Barr encoding region (EBER) in situ hybridization is the methodology of choice for the detection of the Epstein-Barr virus (EBV) in tissue sections. Because of the large numbers of copies of EBERs present in latently infected cells, non-isotopic methods can be used. Positive studies show staining in the nuclei of the EBV-infected cells, accentuating the chromatin and often excluding the nucleolus. False-negative results are most often the result of RNA degradation in the tissues, a finding that may be detected through the use of a polyT probe as a control for RNA preservation.Entities:
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Year: 2013 PMID： 23666702 DOI： 10.1007/978-1-62703-357-2_16
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745