Literature DB >> 23653755

Summer workshop in metagenomics: one week plus eight students equals gigabases of cloned DNA.

Carlos Rios-Velazquez1, Lynn L Williamson, Karen A Cloud-Hansen, Heather K Allen, Mathew D McMahon, Zakee L Sabree, Justin J Donato, Jo Handelsman.   

Abstract

We designed a week-long laboratory workshop in metagenomics for a cohort of undergraduate student researchers. During this course, students learned and utilized molecular biology and microbiology techniques to construct a metagenomic library from Puerto Rican soil. Pre-and postworkshop assessments indicated student learning gains in technical knowledge, skills, and confidence in a research environment. Postworkshop construction of additional libraries demonstrated retention of research techniques by the students.

Year:  2011        PMID: 23653755      PMCID: PMC3577266          DOI: 10.1128/jmbe.v12i2.177

Source DB:  PubMed          Journal:  J Microbiol Biol Educ        ISSN: 1935-7877


  14 in total

1.  Uncultured soil bacteria are a reservoir of new antibiotic resistance genes.

Authors:  Christian S Riesenfeld; Robert M Goodman; Jo Handelsman
Journal:  Environ Microbiol       Date:  2004-09       Impact factor: 5.491

2.  Metagenomic analysis of apple orchard soil reveals antibiotic resistance genes encoding predicted bifunctional proteins.

Authors:  Justin J Donato; Luke A Moe; Brandon J Converse; Keith D Smart; Flora C Berklein; Patricia S McManus; Jo Handelsman
Journal:  Appl Environ Microbiol       Date:  2010-05-07       Impact factor: 4.792

3.  Program targets undergrads to make an early start on science.

Authors:  Emma Marris
Journal:  Nat Med       Date:  2007-11       Impact factor: 53.440

4.  Partners in research: benefits of a summer research program.

Authors:  Thomas Kelly; Elizabeth Ann Coleman; E Kim Fifer; E Robert Burns; Clifton Orr; Richard W Nicholas
Journal:  J Cancer Educ       Date:  2006       Impact factor: 2.037

5.  Recovery, purification, and cloning of high-molecular-weight DNA from soil microorganisms.

Authors:  Mark R Liles; Lynn L Williamson; Jitsupang Rodbumrer; Vigdis Torsvik; Robert M Goodman; Jo Handelsman
Journal:  Appl Environ Microbiol       Date:  2008-03-21       Impact factor: 4.792

6.  The pipeline. Benefits of undergraduate research experiences.

Authors:  Susan H Russell; Mary P Hancock; James McCullough
Journal:  Science       Date:  2007-04-27       Impact factor: 47.728

7.  Resident microbiota of the gypsy moth midgut harbors antibiotic resistance determinants.

Authors:  Heather K Allen; Karen A Cloud-Hansen; Joseph M Wolinski; Changhui Guan; Serena Greene; Shyue Lu; Mallory Boeyink; Nichole A Broderick; Kenneth F Raffa; Jo Handelsman
Journal:  DNA Cell Biol       Date:  2009-03       Impact factor: 3.311

8.  An invitation to the marriage of metagenomics and metabolomics.

Authors:  Peter J Turnbaugh; Jeffrey I Gordon
Journal:  Cell       Date:  2008-09-05       Impact factor: 41.582

9.  Agrobacterium tumefaciens mutants affected in crown gall tumorigenesis and octopine catabolism.

Authors:  D J Garfinkel; E W Nester
Journal:  J Bacteriol       Date:  1980-11       Impact factor: 3.490

Review 10.  Metagenomics for studying unculturable microorganisms: cutting the Gordian knot.

Authors:  Patrick D Schloss; Jo Handelsman
Journal:  Genome Biol       Date:  2005-08-01       Impact factor: 13.583

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  4 in total

1.  Practice makes pretty good: assessment of primary literature reading abilities across multiple large-enrollment biology laboratory courses.

Authors:  Brian K Sato; Pavan Kadandale; Wenliang He; Paige M N Murata; Yama Latif; Mark Warschauer
Journal:  CBE Life Sci Educ       Date:  2014       Impact factor: 3.325

2.  Metagenomic Approaches to Identify Novel Organisms from the Soil Environment in a Classroom Setting.

Authors:  Sadia J Rahman; Trevor C Charles; Parjit Kaur
Journal:  J Microbiol Biol Educ       Date:  2016-12-02

Review 3.  Microbiomes for All.

Authors:  Theodore R Muth; Avrom J Caplan
Journal:  Front Microbiol       Date:  2020-11-12       Impact factor: 5.640

4.  A Systematic Approach To Promote Environmental Engineering Students' Learning in Environmental Molecular Microbiology.

Authors:  Shanquan Wang; Hui Lu; Chunmei Zhao; Philippe Juneau
Journal:  J Microbiol Biol Educ       Date:  2021-03-15
  4 in total

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