OBJECTIVE: We tested the hypothesis that the order of exposure to maternal betamethasone and intra-amniotic (IA) lipopolysaccharide (LPS) will differentially modulate inflammation in the chorioamnion. STUDY DESIGN: Time-mated Merino ewes with singleton fetuses received saline alone, IA LPS alone, maternal betamethasone before LPS, or betamethasone after LPS. We assessed inflammatory markers in the chorioamnion and the amniotic fluid. RESULTS: Inflammatory cell infiltration, expression of myeloperoxidase, serum amyloid A3 (acute phase reactant) in the chorioamnion, and levels of interleukin (IL)-8 in the amniotic fluid increased 7 days after LPS exposure. Betamethasone prior to LPS decreased infiltration of the inflammatory cells, CD3+ T cells, and decreased the levels of IL-1β and IL-8 in the amniotic fluid. CONCLUSIONS: Betamethasone 7 days prior to LPS exposure suppressed LPS-induced inflammation. The markers of inflammation largely had returned to the baseline 14 days after LPS exposure.
OBJECTIVE: We tested the hypothesis that the order of exposure to maternal betamethasone and intra-amniotic (IA) lipopolysaccharide (LPS) will differentially modulate inflammation in the chorioamnion. STUDY DESIGN: Time-mated Merino ewes with singleton fetuses received saline alone, IA LPS alone, maternal betamethasone before LPS, or betamethasone after LPS. We assessed inflammatory markers in the chorioamnion and the amniotic fluid. RESULTS: Inflammatory cell infiltration, expression of myeloperoxidase, serum amyloid A3 (acute phase reactant) in the chorioamnion, and levels of interleukin (IL)-8 in the amniotic fluid increased 7 days after LPS exposure. Betamethasone prior to LPS decreased infiltration of the inflammatory cells, CD3+ T cells, and decreased the levels of IL-1β and IL-8 in the amniotic fluid. CONCLUSIONS:Betamethasone 7 days prior to LPS exposure suppressed LPS-induced inflammation. The markers of inflammation largely had returned to the baseline 14 days after LPS exposure.
Authors: B A Banks; A Cnaan; M A Morgan; J T Parer; J D Merrill; P L Ballard; R A Ballard Journal: Am J Obstet Gynecol Date: 1999-09 Impact factor: 8.661
Authors: Suhas G Kallapur; Boris W Kramer; Timothy J M Moss; John P Newnham; Alan H Jobe; Machiko Ikegami; Cindy J Bachurski Journal: Am J Physiol Lung Cell Mol Physiol Date: 2002-12-06 Impact factor: 5.464
Authors: J P Newnham; T J Moss; J F Padbury; K E Willet; M Ikegami; M G Ervin; P Sly; A Jobe Journal: Am J Obstet Gynecol Date: 2001-07 Impact factor: 8.661
Authors: Boris W Kramer; Machiko Ikegami; Timothy J M Moss; Ilias Nitsos; John P Newnham; Alan H Jobe Journal: Pediatr Res Date: 2004-02-18 Impact factor: 3.756
Authors: John P Newnham; Suhas G Kallapur; Boris W Kramer; Timothy J M Moss; Ilias Nitsos; Machiko Ikegami; Alan H Jobe Journal: Am J Obstet Gynecol Date: 2003-11 Impact factor: 8.661
Authors: Suhas G Kallapur; Pietro Presicce; Cesar M Rueda; Alan H Jobe; Claire A Chougnet Journal: Semin Reprod Med Date: 2014-01-03 Impact factor: 1.303
Authors: Jodi K Regan; Paranthaman S Kannan; Matthew W Kemp; Boris W Kramer; John P Newnham; Alan H Jobe; Suhas G Kallapur Journal: Reprod Sci Date: 2015-07-07 Impact factor: 3.060
Authors: Jennifer Armstrong-Wells; Meghan Donnelly; Miriam D Post; Marilyn J Manco-Johnson; Virginia D Winn; Guillaume Sébire Journal: Am J Obstet Gynecol Date: 2014-09-16 Impact factor: 8.661