| Literature DB >> 23640860 |
Adil Akkouch1, Ze Zhang, Mahmoud Rouabhia.
Abstract
The aim of this study was to design a new natural/synthetic bioactive bone scaffold for potential use in bone replacement applications. We developed a tri-component osteogenic composite scaffold made of collagen (Coll), hydroxyapatite (HA) and poly(l-lactide-co-ε-caprolactone) (PLCL). This Coll/HA/PLCL composite scaffold was combined with human osteoblast-like cells obtained by differentiation of dental pulp stem cells (DPSCs) to engineer bone tissue in vitro. Results show that the 3D Coll/HA/PLCL composite scaffold was highly porous, thereby enabling osteoblast-like cell adhesion and growth. Cultured in the Coll/HA/PLCL scaffold, the osteoblast-like cells expressed different osteogenic genes, produced alkaline phosphatase and formed nodules more than did PLCL alone. Micro-CT analyses revealed a significant (30%) increase of tissue mineralisation on the surface as well as inside of the Coll/HA/PLCL scaffold, thus confirming its effectiveness as a bone regeneration platform.Entities:
Keywords: Human dental pulp stem cells; biomaterial; bone regeneration; collagen; differentiation; hydroxyapatite; poly(lactide-co-ε-caprolactone)
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Year: 2013 PMID： 23640860 DOI： 10.1177/0885328213486705
Source DB: PubMed Journal: J Biomater Appl ISSN： 0885-3282 Impact factor: 2.646