Literature DB >> 2364020

Molecular cloning and characterization of a German HIV-1 isolate.

U Sauermann1, J Schneider, J Mous, U Brunckhorst, I Schedel, K D Jentsch, G Hunsmann.   

Abstract

The genetic diversity of HIV-1 is well documented. Except for the HIV-1 isolate LAV-1BRU, no nucleic acid sequence of a European isolate of HIV-1 has been published to date. To further investigate the extent of the genetic variability and the evolution of HIV-1, we have isolated, cloned, and subsequently sequenced HIV-1 from a German patient with AIDS-related complex. Comparative studies of the nucleic acid sequence revealed that this isolate, designated HAN2, is highly divergent from the North American and African subtypes of HIV-1 and may represent a European subtype of HIV-1. Furthermore, a full-length molecular clone was derived from this isolate which was infectious in human T-cell lines. Therefore this new isolate will be particularly useful for studies on the genetic evolution and biology of HIV-1 as well as for testing antiviral substances and for developing vaccines.

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Year:  1990        PMID： 2364020     DOI： 10.1089/aid.1990.6.813

Source DB:  PubMed          Journal:  AIDS Res Hum Retroviruses        ISSN： 0889-2229            Impact factor:   2.205


  12 in total

1.  Increased neutralization sensitivity and reduced replicative capacity of human immunodeficiency virus type 1 after short-term in vivo or in vitro passage through chimpanzees.

Authors:  T Beaumont; S Broersen; A van Nuenen; H G Huisman; A M de Roda Husman; J L Heeney; H Schuitemaker
Journal:  J Virol       Date:  2000-09       Impact factor: 5.103

2.  HIV-1 in Ethiopia: phylogenetic divergence from other HIV-1 strains.

Authors:  S Ayehunie; B Johansson; M Salminen; P Leinikki; A Sönnerborg; D W Zewdie; S Britton; O Strannegård
Journal:  Virus Genes       Date:  1991-10       Impact factor: 2.332

3.  Activation of NFAT-dependent gene expression by Nef: conservation among divergent Nef alleles, dependence on SH3 binding and membrane association, and cooperation with protein kinase C-theta.

Authors:  A Manninen; P Huotari; M Hiipakka; G H Renkema; K Saksela
Journal:  J Virol       Date:  2001-03       Impact factor: 5.103

4.  Evidence for viral virulence as a predominant factor limiting human immunodeficiency virus vaccine efficacy.

Authors:  P Mooij; W M Bogers; H Oostermeijer; W Koornstra; P J Ten Haaft; B E Verstrepen; G Van Der Auwera; J L Heeney
Journal:  J Virol       Date:  2000-05       Impact factor: 5.103

5.  The rapid spread of recombinants during a natural in vitro infection with two human immunodeficiency virus type 1 strains.

Authors:  T Kuwata; Y Miyazaki; T Igarashi; J Takehisa; M Hayami
Journal:  J Virol       Date:  1997-09       Impact factor: 5.103

6.  V3 loop of the human immunodeficiency virus type 1 Env protein: interpreting sequence variability.

Authors:  L Milich; B Margolin; R Swanstrom
Journal:  J Virol       Date:  1993-09       Impact factor: 5.103

7.  Identification of a subset of human immunodeficiency virus type 1 (HIV-1), HIV-2, and simian immunodeficiency virus strains able to exploit an alternative coreceptor on untransformed human brain and lymphoid cells.

Authors:  Samantha J Willey; Jacqueline D Reeves; Richard Hudson; Koichi Miyake; Nathalie Dejucq; Dominique Schols; Erik De Clercq; Jeanne Bell; Aine McKnight; Paul R Clapham
Journal:  J Virol       Date:  2003-06       Impact factor: 5.103

8.  A pathogenic threshold of virus load defined in simian immunodeficiency virus- or simian-human immunodeficiency virus-infected macaques.

Authors:  P Ten Haaft; B Verstrepen; K Uberla; B Rosenwirth; J Heeney
Journal:  J Virol       Date:  1998-12       Impact factor: 5.103

9.  Primary human immunodeficiency virus type 2 (HIV-2) isolates infect CD4-negative cells via CCR5 and CXCR4: comparison with HIV-1 and simian immunodeficiency virus and relevance to cell tropism in vivo.

Authors:  J D Reeves; S Hibbitts; G Simmons; A McKnight; J M Azevedo-Pereira; J Moniz-Pereira; P R Clapham
Journal:  J Virol       Date:  1999-09       Impact factor: 5.103

10.  Evolution of the primate lentiviruses: evidence from vpx and vpr.

Authors:  M Tristem; C Marshall; A Karpas; F Hill
Journal:  EMBO J       Date:  1992-09       Impact factor: 11.598
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