Literature DB >> 2364018

Expression of HIV-1 gp120 and human soluble CD4 by recombinant baculoviruses and their interaction in vitro.

Y Morikawa1, H A Overton, J P Moore, A J Wilkinson, R L Brady, S J Lewis, I M Jones.   

Abstract

The soluble domains of the envelope glycoprotein of HIV-1 (gp120) and human CD4 (sCD4) have been individually expressed in insect cells using recombinant baculoviruses. Each product is secreted from infected cells and accumulates in the surrounding media to levels of 1-2 mg/liter of 2 x 10(9) cells. Both molecules have full biological activity, and conditioned media from infected cells have been used to establish a simple assay for gp120-sCD4 interaction that is highly specific and amenable to mass screening. The crystallization of sCD4 purified from this source is reported.

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Year:  1990        PMID： 2364018     DOI： 10.1089/aid.1990.6.765

Source DB:  PubMed          Journal:  AIDS Res Hum Retroviruses        ISSN： 0889-2229            Impact factor:   2.205


  18 in total

1.  Host-cell-specific glycosylation of HIV-2 envelope glycoprotein.

Authors:  S Liedtke; R Geyer; H Geyer
Journal:  Glycoconj J       Date:  1997-11       Impact factor: 2.916

2.  Highly active antiretroviral therapy potently suppresses HIV infection in humanized Rag2-/-gammac-/- mice.

Authors:  Kaori Sango; Aviva Joseph; Mahesh Patel; Kristin Osiecki; Monica Dutta; Harris Goldstein
Journal:  AIDS Res Hum Retroviruses       Date:  2010-07       Impact factor: 2.205

3.  Production of multiple murine CD2 receptor constructs using the baculovirus expression vector and a rapid dot-blot assay.

Authors:  A J Kaplan; K D Chavin; L H Qin; H Yagita; J S Bromberg
Journal:  Immunol Res       Date:  1994       Impact factor: 2.829

4.  HIV-1 envelope proteins up-regulate N 6-methyladenosine levels of cellular RNA independently of viral replication.

Authors:  Nagaraja Tirumuru; Li Wu
Journal:  J Biol Chem       Date:  2019-01-07       Impact factor: 5.157

5.  Legitimate and illegitimate cleavage of human immunodeficiency virus glycoproteins by furin.

Authors:  Y Morikawa; E Barsov; I Jones
Journal:  J Virol       Date:  1993-06       Impact factor: 5.103

6.  Interleukin-12 is necessary for the priming of CD4+ T cells required during the elicitation of HIV-1 gp120-specific cytotoxic T-lymphocyte function.

Authors:  Shalini Gupta; Ramanamurthy Boppana; Gyan C Mishra; Bhaskar Saha; Debashis Mitra
Journal:  Immunology       Date:  2008-02-20       Impact factor: 7.397

7.  Functional role of the glycan cluster of the human immunodeficiency virus type 1 transmembrane glycoprotein (gp41) ectodomain.

Authors:  E Fenouillet; I Jones; B Powell; D Schmitt; M P Kieny; J C Gluckman
Journal:  J Virol       Date:  1993-01       Impact factor: 5.103

8.  HIV-1 envelope proteins and V1/V2 domain scaffolds with mannose-5 to improve the magnitude and quality of protective antibody responses to HIV-1.

Authors:  Javier F Morales; Trevor J Morin; Bin Yu; Gwen P Tatsuno; Sara M O'Rourke; Richard Theolis; Kathryn A Mesa; Phillip W Berman
Journal:  J Biol Chem       Date:  2014-07-25       Impact factor: 5.157

9.  Glycosylation is necessary for the correct folding of human immunodeficiency virus gp120 in CD4 binding.

Authors:  Y Li; L Luo; N Rasool; C Y Kang
Journal:  J Virol       Date:  1993-01       Impact factor: 5.103

10.  Regions required for CD4 binding in the external glycoprotein gp120 of simian immunodeficiency virus.

Authors:  C B Doyle; U Bhattacharyya; K A Kent; J E Stott; I M Jones
Journal:  J Virol       Date:  1995-02       Impact factor: 5.103
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