Literature DB >> 2363501

Lowry protein assay containing sodium dodecyl sulfate in microtiter plates for protein determinations on fractions from brain tissue.

C R Harrington1.   

Abstract

A modified Lowry protein assay which contains sodium dodecyl sulfate (Markwell et al., 1978, Anal. Biochem. 87, 207-210) has been adapted for use in 96-well microtiter plates. A spectrophotometer interfaced with a computer is used to plot the standard curve and calculate the protein content of each sample. The method is suitable for measuring 4-40 micrograms of protein/assay in a volume of 50 microliters. There was no need for prior solubilization of proteins which had been extracted from brain tissue. Test samples, up to 21 in triplicate, can be tested on a single microtiter plate plus standards. Alternatively, further samples can be tested separately on extra plates using the standard curve from the original plate for calculations.

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Year:  1990        PMID： 2363501     DOI： 10.1016/0003-2697(90)90081-j

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  16 in total

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2.  Measurement of distinct immunochemical presentations of tau protein in Alzheimer disease.

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Journal:  Proc Natl Acad Sci U S A       Date:  1991-07-01       Impact factor: 11.205

3.  Spectroscopic determination of sarcoplasmic reticulum Ca2+ uptake and Ca2+ release.

Authors:  J S Gilchrist; C Palahniuk; R Bose
Journal:  Mol Cell Biochem       Date:  1997-07       Impact factor: 3.396

4.  Characterization of palmitoylethanolamide transport in mouse Neuro-2a neuroblastoma and rat RBL-2H3 basophilic leukaemia cells: comparison with anandamide.

Authors:  S O Jacobsson; C J Fowler
Journal:  Br J Pharmacol       Date:  2001-04       Impact factor: 8.739

5.  Lack of selectivity of URB602 for 2-oleoylglycerol compared to anandamide hydrolysis in vitro.

Authors:  S Vandevoorde; K-O Jonsson; G Labar; E Persson; D M Lambert; C J Fowler
Journal:  Br J Pharmacol       Date:  2006-12-04       Impact factor: 8.739

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Authors:  S Holt; J Nilsson; R Omeir; G Tiger; C J Fowler
Journal:  Br J Pharmacol       Date:  2001-06       Impact factor: 8.739

7.  Effects of homologues and analogues of palmitoylethanolamide upon the inactivation of the endocannabinoid anandamide.

Authors:  K O Jonsson; S Vandevoorde; D M Lambert; G Tiger; C J Fowler
Journal:  Br J Pharmacol       Date:  2001-08       Impact factor: 8.739

8.  Selective inhibition of Alzheimer disease-like tau aggregation by phenothiazines.

Authors:  C M Wischik; P C Edwards; R Y Lai; M Roth; C R Harrington
Journal:  Proc Natl Acad Sci U S A       Date:  1996-10-01       Impact factor: 11.205

9.  Lysophosphatidic acid inhibits gap-junctional communication and stimulates phosphorylation of connexin-43 in WB cells: possible involvement of the mitogen-activated protein kinase cascade.

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Journal:  Biochem J       Date:  1994-10-15       Impact factor: 3.857

10.  Interleukin-3 facilitates glucose transport in a myeloid cell line by regulating the affinity of the glucose transporter for glucose: involvement of protein phosphorylation in transporter activation.

Authors:  M V Berridge; A S Tan
Journal:  Biochem J       Date:  1995-02-01       Impact factor: 3.857
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