PURPOSE: This study demonstrated invasive and reproducible hepatic artery catheterization in rats. A rigorously documented guide and pictorial essay describes the performance of an invasive hepatic artery catheterization technique suitable for the study of liver-targeted interventional procedures in rodent models of liver cancer. The goal was to produce a well-illustrated guide to hepatic artery catheterization under direct visualization via the gastroduodenal artery (GDA). MATERIALS AND METHODS: 20 Sprague Dawley rats were inoculated with McA-RH7777 HCC cells in the left lateral liver lobe. Magnetic resonance imaging (MRI) was used to measure tumor growth. Catheter placement in the hepatic artery proper was performed by entry through the GDA under direct visualization after laparotomy. Digital subtraction angiography confirmed catheter placement in the hepatic artery proper. Antegrade blood flow to the liver was restored after catheter removal. Rats were euthanized after procedures; livers were harvested for hematoxylin and eosin (H&E) staining. RESULTS: 85.0% of inoculated animals developed measurable tumors on MRI; average tumor size was 6.3 ± 2.3 mm × 4.3 ± 1.5 mm (mean ± SD). 94.1% of animals with tumors were successfully catheterized. H&E staining demonstrated tumor growth in all inoculated animals, including those with no measurable tumors on MRI. CONCLUSION: Invasive catheter placement in the hepatic artery of a rodent model of HCC can be performed reproducibly according to the techniques described in this tutorial. These catheterization techniques are ideal for a broad range of preclinical IR studies intending to evaluate the efficacy of intra-arterial therapies for the treatment of primary and metastatic liver tumors.
PURPOSE: This study demonstrated invasive and reproducible hepatic artery catheterization in rats. A rigorously documented guide and pictorial essay describes the performance of an invasive hepatic artery catheterization technique suitable for the study of liver-targeted interventional procedures in rodent models of liver cancer. The goal was to produce a well-illustrated guide to hepatic artery catheterization under direct visualization via the gastroduodenal artery (GDA). MATERIALS AND METHODS: 20 Sprague Dawley rats were inoculated with McA-RH7777 HCC cells in the left lateral liver lobe. Magnetic resonance imaging (MRI) was used to measure tumor growth. Catheter placement in the hepatic artery proper was performed by entry through the GDA under direct visualization after laparotomy. Digital subtraction angiography confirmed catheter placement in the hepatic artery proper. Antegrade blood flow to the liver was restored after catheter removal. Rats were euthanized after procedures; livers were harvested for hematoxylin and eosin (H&E) staining. RESULTS: 85.0% of inoculated animals developed measurable tumors on MRI; average tumor size was 6.3 ± 2.3 mm × 4.3 ± 1.5 mm (mean ± SD). 94.1% of animals with tumors were successfully catheterized. H&E staining demonstrated tumor growth in all inoculated animals, including those with no measurable tumors on MRI. CONCLUSION: Invasive catheter placement in the hepatic artery of a rodent model of HCC can be performed reproducibly according to the techniques described in this tutorial. These catheterization techniques are ideal for a broad range of preclinical IR studies intending to evaluate the efficacy of intra-arterial therapies for the treatment of primary and metastatic liver tumors.
Entities:
Keywords:
Hepatocellular carcinoma (HCC); McA-RH7777; catheterization; intra-arterial (IA); rodent model
Authors: R J Lewandowski; L M Kulik; A Riaz; S Senthilnathan; M F Mulcahy; R K Ryu; S M Ibrahim; K T Sato; T Baker; F H Miller; R Omary; M Abecassis; R Salem Journal: Am J Transplant Date: 2009-06-22 Impact factor: 8.086
Authors: E A Eisenhauer; P Therasse; J Bogaerts; L H Schwartz; D Sargent; R Ford; J Dancey; S Arbuck; S Gwyther; M Mooney; L Rubinstein; L Shankar; L Dodd; R Kaplan; D Lacombe; J Verweij Journal: Eur J Cancer Date: 2009-01 Impact factor: 9.162
Authors: Jeane Chen; Alexander Y Sheu; Weiguo Li; Zhuoli Zhang; Dong-Hyun Kim; Robert J Lewandowski; Reed A Omary; Lonnie D Shea; Andrew C Larson Journal: J Control Release Date: 2014-04-13 Impact factor: 9.776
Authors: Yuzhu Wang; Junjie Li; Indhumathy Subramaniyan; Goncalo Dias do Vale; Jaideep Chaudhary; Arnida Anwar; Mary Wight-Carter; Jeffrey G McDonald; William C Putnam; Tao Qin; Hongwei Zhang; Ian R Corbin Journal: Toxicol Appl Pharmacol Date: 2020-05-15 Impact factor: 4.219
Authors: Abdul Kareem Parchur; Gayatri Sharma; Jaidip M Jagtap; Venkateswara Rao Gogineni; Peter S LaViolette; Michael J Flister; Sarah Beth White; Amit Joshi Journal: ACS Nano Date: 2018-07-06 Impact factor: 18.027
Authors: Zhanliang Su; Xifu Wang; Linfeng Zheng; Tianchu Lyu; Matteo Figini; Bin Wang; Daniel Procissi; Junjie Shangguan; Chong Sun; Liang Pan; Lei Qin; Bin Zhang; Yury Velichko; Riad Salem; Vahid Yaghmai; Andrew C Larson; Zhuoli Zhang Journal: Cancer Med Date: 2018-03-30 Impact factor: 4.452