| Literature DB >> 23623559 |
Friederike Braulke1, Klaus Jung, Julie Schanz, Katharina Götze, Catharina Müller-Thomas, Uwe Platzbecker, Ulrich Germing, Tim H Brümmendorf, Gesine Bug, Oliver Ottmann, Aristoteles A N Giagounidis, Michael Stadler, Wolf-Karsten Hofmann, Philippe Schafhausen, Michael Lübbert, Richard F Schlenk, Igor W Blau, Christina Ganster, Sebastian Pfeiffer, Katayoon Shirneshan, Michael Metz, Sven Detken, Jörg Seraphin, Kathleen Jentsch-Ullrich, Angelika Böhme, Burkhard Schmidt, Lorenz Trümper, Detlef Haase.
Abstract
The gold standard of cytogenetic analysis in myelodysplastic syndromes (MDS) is conventional chromosome banding (CCB) analysis of bone marrow (BM) metaphases. Most aberrations can also be detected by fluorescence-in situ-hybridization (FISH). For this prospective multicenter German diagnostic study (www.clinicaltrials.gov: #NCT01355913) 360 patients, as yet, were followed up to 3 years by sequential FISH analyses of immunomagnetically enriched CD34+ peripheral blood (PB) cells using comprehensive FISH probe panels, resulting in a total number of 19,516 FISH analyses. We demonstrate that CD34+ PB FISH correlates significantly with CCB analysis and represents a feasible method for a reliable non-invasive cytogenetic monitoring from PB.Entities:
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Year: 2013 PMID： 23623559 DOI： 10.1016/j.leukres.2013.03.019
Source DB: PubMed Journal: Leuk Res ISSN： 0145-2126 Impact factor: 3.156