Literature DB >> 23616455

Integrating the Xpert MTB/RIF assay into a diagnostic workflow for rapid detection of Mycobacterium tuberculosis in a low-prevalence area.

Vanessa Deggim1, Akos Somoskovi, Antje Voit, Erik C Böttger, Guido V Bloemberg.   

Abstract

The Xpert MTB/RIF assay is a rapid and fully automated real-time PCR assay. The performance of the Xpert MTB/RIF assay as a primary screening test for urgent clinical specimens was evaluated during a 2-year period. The results showed that replacing smear microscopy with the Xpert MTB/RIF assay facilitates laboratory handling and improves the sensitivity and specificity of Mycobacterium tuberculosis detection.

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Year:  2013        PMID： 23616455      PMCID： PMC3697699          DOI： 10.1128/JCM.00151-13

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  19 in total

Review 1.  Relevance of commercial amplification methods for direct detection of Mycobacterium tuberculosis complex in clinical samples.

Authors:  Claudio Piersimoni; Claudio Scarparo
Journal:  J Clin Microbiol       Date:  2003-12       Impact factor: 5.948

2.  Line probe and automated real-time PCR TB assays warrant adherence to strict quality assurance measures.

Authors:  Akos Somoskovi; Linda M Parsons; Cristina Gutierrez; Evan Lee; Max Salfinger
Journal:  Int J Tuberc Lung Dis       Date:  2012-05       Impact factor: 2.373

Review 3.  Optimizing sputum smear microscopy for the diagnosis of pulmonary tuberculosis.

Authors:  Karen R Steingart; Andrew Ramsay; Madhukar Pai
Journal:  Expert Rev Anti Infect Ther       Date:  2007-06       Impact factor: 5.091

4.  Differentiation of Mycobacterium species by direct sequencing of amplified DNA.

Authors:  T Rogall; T Flohr; E C Böttger
Journal:  J Gen Microbiol       Date:  1990-09

5.  Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clinical laboratory.

Authors:  P Kirschner; B Springer; U Vogel; A Meier; A Wrede; M Kiekenbeck; F C Bange; E C Böttger
Journal:  J Clin Microbiol       Date:  1993-11       Impact factor: 5.948

6.  Clinical evaluation of the automated COBAS AMPLICOR MTB assay for testing respiratory and nonrespiratory specimens.

Authors:  U Reischl; N Lehn; H Wolf; L Naumann
Journal:  J Clin Microbiol       Date:  1998-10       Impact factor: 5.948

7.  Two-laboratory collaborative study on identification of mycobacteria: molecular versus phenotypic methods.

Authors:  B Springer; L Stockman; K Teschner; G D Roberts; E C Böttger
Journal:  J Clin Microbiol       Date:  1996-02       Impact factor: 5.948

8.  Diagnosis of mycobacterial infections by nucleic acid amplification: 18-month prospective study.

Authors:  P Kirschner; J Rosenau; B Springer; K Teschner; K Feldmann; E C Böttger
Journal:  J Clin Microbiol       Date:  1996-02       Impact factor: 5.948

9.  Detection and identification of mycobacteria by amplification of rRNA.

Authors:  B Böddinghaus; T Rogall; T Flohr; H Blöcker; E C Böttger
Journal:  J Clin Microbiol       Date:  1990-08       Impact factor: 5.948

10.  Quantitative drug susceptibility testing of Mycobacterium tuberculosis by use of MGIT 960 and EpiCenter instrumentation.

Authors:  Burkhard Springer; Katja Lucke; Romana Calligaris-Maibach; Claudia Ritter; Erik C Böttger
Journal:  J Clin Microbiol       Date:  2009-04-01       Impact factor: 5.948
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  13 in total

1.  Reply to "performance of the Xpert MTB/RIF assay on nonrespiratory specimens and accuracy of this assay for detection of rifampin resistance in a low-prevalence setting".

Authors:  Vanessa Deggim; Akos Somoskovi; Guido Bloemberg
Journal:  J Clin Microbiol       Date:  2014-02       Impact factor: 5.948

2.  Performance of the Xpert MTB/RIF assay on nonrespiratory specimens and accuracy of this assay for detection of rifampin resistance in a low-prevalence setting.

Authors:  Annette Blaich; Reno Frei
Journal:  J Clin Microbiol       Date:  2014-02       Impact factor: 5.948

3.  Xpert MTB/RIF Ultra and Xpert MTB/RIF assays for extrapulmonary tuberculosis and rifampicin resistance in adults.

Authors:  Mikashmi Kohli; Ian Schiller; Nandini Dendukuri; Mandy Yao; Keertan Dheda; Claudia M Denkinger; Samuel G Schumacher; Karen R Steingart
Journal:  Cochrane Database Syst Rev       Date:  2021-01-15

Review 4.  Evaluation of the Cepheid Xpert MTB/RIF assay.

Authors:  Thomas M Shinnick; Angela M Starks; Heather L Alexander; Kenneth G Castro
Journal:  Expert Rev Mol Diagn       Date:  2014-11-06       Impact factor: 5.225

5.  Xpert MTB/RIF and Xpert MTB/RIF Ultra for pulmonary tuberculosis and rifampicin resistance in adults.

Authors:  David J Horne; Mikashmi Kohli; Jerry S Zifodya; Ian Schiller; Nandini Dendukuri; Deanna Tollefson; Samuel G Schumacher; Eleanor A Ochodo; Madhukar Pai; Karen R Steingart
Journal:  Cochrane Database Syst Rev       Date:  2019-06-07

Review 6.  Xpert® MTB/RIF assay for extrapulmonary tuberculosis and rifampicin resistance.

Authors:  Mikashmi Kohli; Ian Schiller; Nandini Dendukuri; Keertan Dheda; Claudia M Denkinger; Samuel G Schumacher; Karen R Steingart
Journal:  Cochrane Database Syst Rev       Date:  2018-08-27

Review 7.  Diagnostic accuracy of the Xpert MTB/RIF assay for extrapulmonary and pulmonary tuberculosis when testing non-respiratory samples: a systematic review.

Authors:  Laura Maynard-Smith; Natasha Larke; Jurgens A Peters; Stephen D Lawn
Journal:  BMC Infect Dis       Date:  2014-12-31       Impact factor: 3.090

8.  Performance evaluation of the Xpert MTB/RIF assay according to its clinical application.

Authors:  Hee Jae Huh; Byeong-Ho Jeong; Kyeongman Jeon; Won-Jung Koh; Chang-Seok Ki; Nam Yong Lee
Journal:  BMC Infect Dis       Date:  2014-11-14       Impact factor: 3.090

9.  Evaluation of GeneXpert MTB/RIF assay for direct diagnosis of pulmonary tuberculosis.

Authors:  Husseiny Sh Moussa; Faten S Bayoumi; Ahmed M Ali
Journal:  Saudi Med J       Date:  2016-10       Impact factor: 1.484

10.  Sensitivity and specificity of Cobas TaqMan MTB real-time polymerase chain reaction for culture-proven Mycobacterium tuberculosis: meta-analysis of 26999 specimens from 17 Studies.

Authors:  Nobuyuki Horita; Masaki Yamamoto; Takashi Sato; Toshinori Tsukahara; Hideyuki Nagakura; Ken Tashiro; Yuji Shibata; Hiroki Watanabe; Kenjiro Nagai; Kentaro Nakashima; Ryota Ushio; Misako Ikeda; Kentaro Sakamaki; Takashi Yoshiyama; Takeshi Kaneko
Journal:  Sci Rep       Date:  2015-12-09       Impact factor: 4.379
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