Development of a method for the determination of citrinin in barley, rye and wheat by solid phase extraction on aminopropyl columns and HPLC-FLD.

A method for the determination of the mycotoxin citrinin (CT) in rye, wheat and barley is described. The proposed method is based on ethyl acetate extraction, solid phase clean-up (SPE) on aminopropyl columns and reversed phase high performance liquid chromatography with fluorescence detection (RP-HPLC-FLD). The limits of detection and quantification of CT amounted to 0.6-0.9 μg/kg and 1.7- 3.3 μg/kg with mean recovery rates in the range of 77-92% (RSD 4.8-5.5%). This method can also be used for the determination of CT in red-fermented rice.