Biosynthesis, isolation, purification and separation of nivalenol, fusarenone - X and zearalenone.

Fusarium, graminearum KF 370 isolate is able to simultaneous biosynthesis of three toxic metabolites, namely: fusarenone-X (FUS), nivalenol (NIV) and zearalenone (F-2). After metabolites extraction with methanol - water (3:1) and defatting with n-heptane toxins were partitioned into chloroform layer. Purification of the? compounds was performed on Celite 545 - charcoal - Aluminiumoxid 90 column then metabolites were separated on Kieselgel 60 (200-300 mesh) column with developing solvent chloroform - methanol. This way FUS, NIV and F-2 were obtained as crystalline or high purity standards.