Literature DB >> 23605390

Simple and efficient cell disruption of extremely small quantities of mycelium of phytopathogenic mycotoxin-producing moulds for quantitative extraction of genomic DNA.

V Saß1, J Milles, J Krämer, A Prange.   

Abstract

DNA was efficiently and quantitatively isolated from extremely small quantities of mycelia (0.1-10 mg) of different phytopathogenic moulds by grinding freeze-dried mycelia with glass beads and then using a commercial DNA extraction kit. The efficiency of disruption of the mycelia and the quantitative DNA extraction was proved by microscopy and the quantification of isolated DNA by real time PCR.

Year:  2005        PMID： 23605390     DOI： 10.1007/BF02957580

Source DB:  PubMed          Journal:  Mycotoxin Res        ISSN： 0178-7888            Impact factor:   3.833


  9 in total

1.  Comparison of six extraction techniques for isolation of DNA from filamentous fungi.

Authors:  J A van Burik; R W Schreckhise; T C White; R A Bowden; D Myerson
Journal:  Med Mycol       Date:  1998-10       Impact factor: 4.076

2.  Evaluation of rapid DNA extraction methods for the quantitative detection of fungi using real-time PCR analysis.

Authors:  Richard A Haugland; Nichole Brinkman; Stephen J Vesper
Journal:  J Microbiol Methods       Date:  2002-08       Impact factor: 2.363

3.  In vivo monitoring of obligate biotrophic pathogen growth by kinetic PCR.

Authors:  Brian Boyle; Richard C Hamelin; Armand Séguin
Journal:  Appl Environ Microbiol       Date:  2005-03       Impact factor: 4.792

4.  Rapid and efficient extraction of genomic DNA from different phytopathogenic fungi using DNAzol reagent.

Authors:  Jian-Rong Guo; F Schnieder; K A Abd-Elsalam; J A Verreet
Journal:  Biotechnol Lett       Date:  2005-01       Impact factor: 2.461

5.  Rapid extraction of fungal DNA for PCR amplification.

Authors:  J L Cenis
Journal:  Nucleic Acids Res       Date:  1992-05-11       Impact factor: 16.971

6.  Improved methods for the preparation of high molecular weight DNA from large and small scale cultures of filamentous fungi.

Authors:  B W Bainbridge; C L Spreadbury; F G Scalise; J Cohen
Journal:  FEMS Microbiol Lett       Date:  1990-01-01       Impact factor: 2.742

7.  Real time detection of the tri5 gene in Fusarium species by lightcycler-PCR using SYBR Green I for continuous fluorescence monitoring.

Authors:  H Schnerr; L Niessen; R F Vogel
Journal:  Int J Food Microbiol       Date:  2001-12-04       Impact factor: 5.277

8.  Detection of Alternaria fungal contamination in cereal grains by a polymerase chain reaction-based assay.

Authors:  Gideon Zur; Eyal Shimoni; Eric Hallerman; Yechezkel Kashi
Journal:  J Food Prot       Date:  2002-09       Impact factor: 2.077

9.  Real-time PCR monitoring of fungal development in Arabidopsis thaliana infected by Alternaria brassicicola and Botrytis cinerea.

Authors:  Claire Gachon; Patrick Saindrenan
Journal:  Plant Physiol Biochem       Date:  2004-05       Impact factor: 4.270
  9 in total

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