Literature DB >> 12031583

Evaluation of rapid DNA extraction methods for the quantitative detection of fungi using real-time PCR analysis.

Richard A Haugland1, Nichole Brinkman, Stephen J Vesper.   

Abstract

Three comparatively rapid methods for the extraction of DNA from fungal conidia and yeast cells in environmental (air, water and dust) samples were evaluated for use in real-time PCR (TaqMan) analyses. A simple bead milling method was developed to provide sensitive, accurate and precise quantification of target organisms in air and water (tap and surface) samples. However, quantitative analysis of dust samples required further purification of the extracted DNA by a streamlined silica adsorption procedure.

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Year:  2002        PMID： 12031583     DOI： 10.1016/s0167-7012(02)00037-4

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  36 in total

1.  Evaluation of a rapid, quantitative real-time PCR method for enumeration of pathogenic Candida cells in water.

Authors:  Nichole E Brinkman; Richard A Haugland; Larry J Wymer; Muruleedhara Byappanahalli; Richard L Whitman; Stephen J Vesper
Journal:  Appl Environ Microbiol       Date:  2003-03       Impact factor: 4.792

2.  Specificity of a Bacteroides thetaiotaomicron marker for human feces.

Authors:  C Andrew Carson; Jessica M Christiansen; Helen Yampara-Iquise; Verel W Benson; Claire Baffaut; Jerri V Davis; Robert R Broz; William B Kurtz; Wendi M Rogers; William H Fales
Journal:  Appl Environ Microbiol       Date:  2005-08       Impact factor: 4.792

3.  Relative moldiness index as predictor of childhood respiratory illness.

Authors:  Stephen J Vesper; Craig McKinstry; Richard A Haugland; Yulia Iossifova; Grace Lemasters; Linda Levin; Gurjit K Khurana Hershey; Manuel Villareal; David I Bernstein; James Lockey; Tiina Reponen
Journal:  J Expo Sci Environ Epidemiol       Date:  2006-10-11       Impact factor: 5.563

4.  Some chronic rhinosinusitis patients have elevated populations of fungi in their sinuses.

Authors:  Andrew H Murr; Andrew N Goldberg; Steven D Pletcher; Kelsey Dillehay; Larry J Wymer; Stephen J Vesper
Journal:  Laryngoscope       Date:  2012-04-24       Impact factor: 3.325

5.  Analysis of fungal flora in indoor dust by ribosomal DNA sequence analysis, quantitative PCR, and culture.

Authors:  M Pitkäranta; T Meklin; A Hyvärinen; L Paulin; P Auvinen; A Nevalainen; H Rintala
Journal:  Appl Environ Microbiol       Date:  2007-11-02       Impact factor: 4.792

6.  Comparison of mold concentrations quantified by MSQPCR in indoor and outdoor air sampled simultaneously.

Authors:  Teija Meklin; Tiina Reponen; Craig McKinstry; Seung-Hyun Cho; Sergey A Grinshpun; Aino Nevalainen; Asko Vepsäläinen; Richard A Haugland; Grace Lemasters; Stephen J Vesper
Journal:  Sci Total Environ       Date:  2007-04-30       Impact factor: 7.963

7.  Correlation between ERMI values and other moisture and mold assessments of homes in the American Healthy Homes Survey.

Authors:  Stephen Vesper; Craig McKinstry; David Cox; Gary Dewalt
Journal:  J Urban Health       Date:  2009-11       Impact factor: 3.671

8.  Comparison of six DNA extraction methods for recovery of fungal DNA as assessed by quantitative PCR.

Authors:  David N Fredricks; Caitlin Smith; Amalia Meier
Journal:  J Clin Microbiol       Date:  2005-10       Impact factor: 5.948

9.  Comparison of indoor air sampling and dust collection methods for fungal exposure assessment using quantitative PCR.

Authors:  Jennie Cox; Reshmi Indugula; Stephen Vesper; Zheng Zhu; Roman Jandarov; Tiina Reponen
Journal:  Environ Sci Process Impacts       Date:  2017-10-18       Impact factor: 4.238

10.  Species-specific fungal DNA in airborne dust as surrogate for occupational mycotoxin exposure?

Authors:  Anne Straumfors Halstensen
Journal:  Int J Mol Sci       Date:  2008-12-10       Impact factor: 6.208
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