Simultaneous analysis of beauvericin and moniliformin in fungal cultures and in cereal grain samples.

Species ofFusarium subglutinans andFusarium proliferation have been found to produce two mycotoxins, beauvericin and moniliformin, under labolatory conditions as well as in infected ears. A method for simultaneous extraction, analysis and quantitation of both metabolites was elaborated. Recoveries were 85-97 % and 78-94 % for the first and the latter mycotoxin, respectively. Detection limit of beauvericin on high- performance thin- layer chromatography plates (Merck 5633) after exposure to iodine vapours was 3 μg/g and by high- performance liquid chromatography method 0.07 μg/g while moniliformin was analyzed at concentration level 1 μg/g by thin- layer chromatography and 0.05 μg/g by high- performance liquid chromatography method.