Literature DB >> 23601327

Assessment of 0.5 T static field exposure effect on yeast and HEK cells using electrorotation.

Amal El-Gaddar1, M Frénéa-Robin, D Voyer, H Aka, N Haddour, L Krähenbühl.   

Abstract

This study aims to examine the influence of a 0.5 T static magnetic field (SMF) on yeast and human embryonic kidney (HEK) 293 cell using electrorotation (ROT). Following 48 h exposition to the SMF, no difference was noted between ROT spectra of unexposed and exposed yeast cells, which extend previous reports on the absence of SMF effects on yeast. We also compared the ROT spectrum and the extracted electrical characteristics of HEK cells exposed during 72 h to a 0.5 T uniform static magnetic field with those of unexposed cells. SMF potential effects on HEK proliferation kinetics and cell morphology were also assessed by using the trypan blue exclusion method and scanning electron microscopy, respectively. At last, no significant differences were observed between control and exposed HEK cells concerning electrical properties, growth, and morphology.
Copyright © 2013 Biophysical Society. Published by Elsevier Inc. All rights reserved.

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Year:  2013        PMID: 23601327      PMCID: PMC3628304          DOI: 10.1016/j.bpj.2013.01.063

Source DB:  PubMed          Journal:  Biophys J        ISSN: 0006-3495            Impact factor:   4.033


  35 in total

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Review 3.  Safety of strong, static magnetic fields.

Authors:  J F Schenck
Journal:  J Magn Reson Imaging       Date:  2000-07       Impact factor: 4.813

4.  Cell shape and plasma membrane alterations after static magnetic fields exposure.

Authors:  A Chionna; M Dwikat; E Panzarini; B Tenuzzo; E C Carlà; T Verri; P Pagliara; L Abbro; L Dini
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Review 5.  Bioeffects of moderate-intensity static magnetic fields on cell cultures.

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Journal:  Micron       Date:  2005-01-25       Impact factor: 2.251

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Journal:  J Membr Biol       Date:  2008-01-16       Impact factor: 1.843

7.  Alterations of the plasma membrane caused by murine polyomavirus proliferation: an electrorotation study.

Authors:  Valerio Berardi; Cecilia Aiello; Adalberto Bonincontro; Gianfranco Risuleo
Journal:  J Membr Biol       Date:  2009-05-09       Impact factor: 1.843

8.  Automatic cell electrorotation measurements: studies of the biological effects of low-frequency magnetic fields and of heat shock.

Authors:  X F Zhou; J P Burt; R Pethig
Journal:  Phys Med Biol       Date:  1998-05       Impact factor: 3.609

9.  Non-uniform spatial distributions of both the magnitude and phase of AC electric fields determine dielectrophoretic forces.

Authors:  X B Wang; M P Hughes; Y Huang; F F Becker; P R Gascoyne
Journal:  Biochim Biophys Acta       Date:  1995-02-23

10.  Dielectrophoretic studies of the activation of human T lymphocytes using a newly developed cell profiling system.

Authors:  Ronald Pethig; Vincent Bressler; Catherine Carswell-Crumpton; Yan Chen; Linda Foster-Haje; Marcos E García-Ojeda; Richard S Lee; Gary M Lock; Mark S Talary; Keri M Tate
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  4 in total

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2.  Accurate Extraction of the Self-Rotational Speed for Cells in an Electrokinetics Force Field by an Image Matching Algorithm.

Authors:  Xieliu Yang; Xihui Niu; Zhu Liu; Yuliang Zhao; Guanglie Zhang; Wenfeng Liang; Wen Jung Li
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3.  On-chip technology for single-cell arraying, electrorotation-based analysis and selective release.

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Journal:  Electrophoresis       Date:  2019-06-03       Impact factor: 3.535

Review 4.  Determination of Dielectric Properties of Cells using AC Electrokinetic-based Microfluidic Platform: A Review of Recent Advances.

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  4 in total

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