BACKGROUND: We evaluated the immunological responses of African green monkeys immunized with multiple F and G protein-based vaccines and assessed protection against the Memphis 37 strain of respiratory syncytial virus (RSV). METHODS: Monkeys were immunized with F and G proteins adjuvanted with immunostimulatory (CpG) oligodeoxyribonucleotides admixed with either Alhydrogel or ISCOMATRIX adjuvant. Delivery of F and G proteins via replication incompetent recombinant vesicular stomatitis viruses (VSVs) and human adenoviruses was also evaluated. Mucosally or parenterally administered recombinant adenoviruses were used in prime-boost regimens with adjuvanted proteins or recombinant DNA. RESULTS: Animals primed by intranasal delivery of recombinant adenoviruses, and boosted by intramuscular injection of adjuvanted F and G proteins, developed neutralizing antibodies and F/G protein-specific T cells and were protected from RSV infection. Intramuscular injections of Alhydrogel (plus CpG) adjuvanted F and G proteins reduced peak viral loads in the lungs of challenged monkeys. Granulocyte numbers were not significantly elevated, relative to controls, in postchallenge bronchoalveolar lavage samples from vaccinated animals. CONCLUSIONS: This study has validated the use of RSV (Memphis 37) in an African green monkey model of intranasal infection and identified nonreplicating vaccines capable of eliciting protection in this higher species challenge model.
BACKGROUND: We evaluated the immunological responses of African green monkeys immunized with multiple F and G protein-based vaccines and assessed protection against the Memphis 37 strain of respiratory syncytial virus (RSV). METHODS: Monkeys were immunized with F and G proteins adjuvanted with immunostimulatory (CpG) oligodeoxyribonucleotides admixed with either Alhydrogel or ISCOMATRIX adjuvant. Delivery of F and G proteins via replication incompetent recombinant vesicular stomatitis viruses (VSVs) and human adenoviruses was also evaluated. Mucosally or parenterally administered recombinant adenoviruses were used in prime-boost regimens with adjuvanted proteins or recombinant DNA. RESULTS: Animals primed by intranasal delivery of recombinant adenoviruses, and boosted by intramuscular injection of adjuvanted F and G proteins, developed neutralizing antibodies and F/G protein-specific T cells and were protected from RSV infection. Intramuscular injections of Alhydrogel (plus CpG) adjuvanted F and G proteins reduced peak viral loads in the lungs of challenged monkeys. Granulocyte numbers were not significantly elevated, relative to controls, in postchallenge bronchoalveolar lavage samples from vaccinated animals. CONCLUSIONS: This study has validated the use of RSV (Memphis 37) in an African green monkey model of intranasal infection and identified nonreplicating vaccines capable of eliciting protection in this higher species challenge model.
Authors: Robert Jordan; Matt Shao; Richard L Mackman; Michel Perron; Tomas Cihlar; Sandy A Lewis; Eugene J Eisenberg; Anne Carey; Robert G Strickley; Jason W Chien; Mark L Anderson; Heather A McEligot; Nicole E Behrens; Laurel J Gershwin Journal: Antimicrob Agents Chemother Date: 2015-06-08 Impact factor: 5.191
Authors: Young-In Kim; John P DeVincenzo; Bart G Jones; Rajeev Rudraraju; Lisa Harrison; Rachel Meyers; Jeff Cehelsky; Rene Alvarez; Julia L Hurwitz Journal: PLoS One Date: 2014-11-21 Impact factor: 3.240
Authors: Hualin Li; Cheryl Callahan; Michael Citron; Zhiyun Wen; Sinoeun Touch; Morgan A Monslow; Kara S Cox; Daniel J DiStefano; Kalpit A Vora; Andrew Bett; Amy Espeseth Journal: PLoS One Date: 2017-11-09 Impact factor: 3.240
Authors: Duncan C Humphries; Richard A O'Connor; Daniel Larocque; Martine Chabaud-Riou; Kevin Dhaliwal; Vincent Pavot Journal: Front Immunol Date: 2021-09-17 Impact factor: 7.561