OBJECTIVES: Silver nanoparticles (AgNPs) with a size ranging from 7 to 70 nm were synthesized using the ascorbic acid-citrate seed-mediated growth approach at room temperature. METHODS: The 8 nm silver particles were prepared using gallic acid in alkaline conditions and used as seed to prepare AgNPs. RESULTS: The presence of ascorbic acid and citrate allows the regulation of size and size distribution of the nanoparticles. The increase in free silver ion-to-seed ratio (Ag(+)/Ag(0)) resulted in changes of particle shape from spherical to pseudo-spherical and minor cylindrical shape. Further, a repetitive seeding approach resulted in the formation of pseudo-spherical particles with higher polydispersity index and minor distributions of tetrahedral particles. Citrate-capped AgNPs were stable and did not agglomerate upon centrifugation. The effect of AgNPs on biofilm reduction was evaluated using static culture on 96-well microtiter plates. Results showed that AgNPs with the smallest average diameter were most effective in the reduction of Pseudomonas aeruginosa biofilm colonies, which accounted for 90% of removal. CONCLUSION: The biofilm removal activities of the nanoparticles were found to be concentration-independent particularly for the concentration within the range of 80-200 µg/mL.
OBJECTIVES:Silver nanoparticles (AgNPs) with a size ranging from 7 to 70 nm were synthesized using the ascorbic acid-citrate seed-mediated growth approach at room temperature. METHODS: The 8 nm silver particles were prepared using gallic acid in alkaline conditions and used as seed to prepare AgNPs. RESULTS: The presence of ascorbic acid and citrate allows the regulation of size and size distribution of the nanoparticles. The increase in free silver ion-to-seed ratio (Ag(+)/Ag(0)) resulted in changes of particle shape from spherical to pseudo-spherical and minor cylindrical shape. Further, a repetitive seeding approach resulted in the formation of pseudo-spherical particles with higher polydispersity index and minor distributions of tetrahedral particles. Citrate-capped AgNPs were stable and did not agglomerate upon centrifugation. The effect of AgNPs on biofilm reduction was evaluated using static culture on 96-well microtiter plates. Results showed that AgNPs with the smallest average diameter were most effective in the reduction of Pseudomonas aeruginosa biofilm colonies, which accounted for 90% of removal. CONCLUSION: The biofilm removal activities of the nanoparticles were found to be concentration-independent particularly for the concentration within the range of 80-200 µg/mL.
Entities:
Keywords:
Antibacterial; Pseudomonas aeruginosa; biofilm; chemical reduction; silver nanoparticles
Authors: Marc B Habash; Amber J Park; Emily C Vis; Robert J Harris; Cezar M Khursigara Journal: Antimicrob Agents Chemother Date: 2014-07-21 Impact factor: 5.191
Authors: Marc B Habash; Mara C Goodyear; Amber J Park; Matthew D Surette; Emily C Vis; Robert J Harris; Cezar M Khursigara Journal: Antimicrob Agents Chemother Date: 2017-10-24 Impact factor: 5.191