| Literature DB >> 23585095 |
Irene Zubiri1, Fernando Vivanco, Gloria Alvarez-Llamas.
Abstract
Urinary exosomes are membranous vesicles 40-100 nm in size containing proteins that are characteristic of every renal tubule epithelial cell type. In this chapter, we describe a methodology to isolate and analyze urinary exosomes proteome by 2-DE and LC-MS/MS, in the search for biomarkers of vascular and associated kidney diseases. We describe an isolation methodology by serial (ultra)centrifugation steps compatible with 2-DE and LC-MS/MS analysis. Exosome purity is confirmed by electron microscopy and Western blot.Entities:
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Year: 2013 PMID： 23585095 DOI： 10.1007/978-1-62703-405-0_16
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745