BACKGROUND: This study was conducted to estimate the prevalence of Plasmodium vivax and polymorphisms in the Duffy antigen receptor for chemokines (DARC) gene in patients with suspected malaria from eastern (Harar) and southwestern (Jimma) Ethiopia. METHODS: Plasmodium presence and species was assessed by microscopy in 1304 and 627 febrile patients in Harar and Jimma, respectively, during October-November 2009. All microscopy-positive samples were confirmed by PCR. DARC gene polymorphisms were identified by DNA sequencing. RESULTS: Plasmodium vivax was the dominant species in Harar (74/98, 76%) and P. falciparum was more common in Jimma (70/107, 65%). We found 17/98 (17%) and 24/107 (22%) homozygous Duffy-negative patients in Harar and Jimma, respectively. Unexpectedly, three Duffy-negative patients from Harar had P. vivax malaria. CONCLUSION: This study documents the emergence of P. vivax malaria in Duffy-negative individuals in Ethiopia. The Duffy-negative blood group does not appear to provide absolute protection against P. vivax infection in this region.
BACKGROUND: This study was conducted to estimate the prevalence of Plasmodium vivax and polymorphisms in the Duffy antigen receptor for chemokines (DARC) gene in patients with suspected malaria from eastern (Harar) and southwestern (Jimma) Ethiopia. METHODS:Plasmodium presence and species was assessed by microscopy in 1304 and 627 febrile patients in Harar and Jimma, respectively, during October-November 2009. All microscopy-positive samples were confirmed by PCR. DARC gene polymorphisms were identified by DNA sequencing. RESULTS:Plasmodium vivax was the dominant species in Harar (74/98, 76%) and P. falciparum was more common in Jimma (70/107, 65%). We found 17/98 (17%) and 24/107 (22%) homozygous Duffy-negative patients in Harar and Jimma, respectively. Unexpectedly, three Duffy-negative patients from Harar had P. vivaxmalaria. CONCLUSION: This study documents the emergence of P. vivaxmalaria in Duffy-negative individuals in Ethiopia. The Duffy-negative blood group does not appear to provide absolute protection against P. vivaxinfection in this region.
Authors: Rachel F Daniels; Benjamin L Rice; Noah M Daniels; Sarah K Volkman; Daniel L Hartl Journal: Pathog Glob Health Date: 2015-04-18 Impact factor: 2.894
Authors: Nicholas F Brazeau; Amy N Whitesell; Stephanie M Doctor; Corinna Keeler; Melchior Kashamuka Mwandagalirwa; Antoinette K Tshefu; Joris L Likwela; Jonathan J Juliano; Steven R Meshnick Journal: Am J Trop Med Hyg Date: 2018-11 Impact factor: 2.345
Authors: Jakub Gruszczyk; Usheer Kanjee; Li-Jin Chan; Sébastien Menant; Benoit Malleret; Nicholas T Y Lim; Christoph Q Schmidt; Yee-Foong Mok; Kai-Min Lin; Richard D Pearson; Gabriel Rangel; Brian J Smith; Melissa J Call; Michael P Weekes; Michael D W Griffin; James M Murphy; Jonathan Abraham; Kanlaya Sriprawat; Maria J Menezes; Marcelo U Ferreira; Bruce Russell; Laurent Renia; Manoj T Duraisingh; Wai-Hong Tham Journal: Science Date: 2018-01-05 Impact factor: 47.728