Literature DB >> 23572917

A reliable protocol for transformation of Catharanthus roseus through Agrobacterium tumefaciens.

Toolika Srivastava1, Sandip Das, Sudhir Kumar Sopory, P S Srivastava.   

Abstract

Proliferation of axillary shoot buds and multiple shoot formation in Catharanthus roseus was obtained in 96 % explants on MS medium (3 % sucrose) containing NAA + BA. 2,4-D induced callusing in both, the nodal as well as in leaf segments. Leaf-derived callus was used for transformation with Agrobacterium tumefaciens LBA4404/pBI-S1. Bacterial cell concentration, duration of co-cultivation and acetosyringone concentration influenced transformation efficiency. Under optimal co-cultivation conditions, 98 % of the explants showed GUS expression. PCR based amplification of the transformed and subsequently selected callus tissue indicated the presence of uidA, Gly I and nptII genes.

Entities:  

Keywords:  Agrobacterium tumefaciens; Catharanthus roseus; Co-cultivation; GUS; GlyI; Micropropagation; RAPD; Transformation

Year:  2009        PMID： 23572917      PMCID： PMC3550379          DOI： 10.1007/s12298-009-0010-1

Source DB:  PubMed          Journal:  Physiol Mol Biol Plants        ISSN： 0974-0430


  11 in total

1.  Plant regeneration from hairy-root cultures transformed by infection with Agrobacterium rhizogenes in Catharanthus roseus.

Authors:  P S Choi; Y D Kim; K M Choi; H J Chung; D W Choi; J R Liu
Journal:  Plant Cell Rep       Date:  2004-02-13       Impact factor: 4.570

2.  Glyoxalase I from Brassica juncea: molecular cloning, regulation and its over-expression confer tolerance in transgenic tobacco under stress.

Authors:  V S Reddy; S K Sopory
Journal:  Plant J       Date:  1999-02       Impact factor: 6.417

3.  Relationship between cell morphology and indole alkaloid production in suspension cultures of Catharanthus roseus.

Authors:  S W Kim; K H Jung; S S Kwak; J R Liu
Journal:  Plant Cell Rep       Date:  1994-11       Impact factor: 4.570

4.  Growth and terpenoid indole alkaloid production in Catharanthus roseus hairy root clones in relation to left- and right-termini-linked Ri T-DNA gene integration.

Authors:  Jyoti Batra; Ajaswrata Dutta; Digvijay Singh; Sushil Kumar; Jayanti Sen
Journal:  Plant Cell Rep       Date:  2004-06-25       Impact factor: 4.570

5.  Factors influencing Agrobacterium-mediated transient expression of gusA in rice.

Authors:  X Q Li; C N Liu; S W Ritchie; J Y Peng; S B Gelvin; T K Hodges
Journal:  Plant Mol Biol       Date:  1992-12       Impact factor: 4.076

6.  Production of indole alkaloids by selected hairy root lines of Catharanthus roseus.

Authors:  R Bhadra; S Vani; J V Shanks
Journal:  Biotechnol Bioeng       Date:  1993-03-05       Impact factor: 4.530

7.  Effects of over-expression of strictosidine synthase and tryptophan decarboxylase on alkaloid production by cell cultures of Catharanthus roseus.

Authors:  C Canel; M I Lopes-Cardoso; S Whitmer; L van der Fits; G Pasquali; R van der Heijden; J H Hoge; R Verpoorte
Journal:  Planta       Date:  1998-07       Impact factor: 4.116

8.  Alkaloid formation by habituated and tumorous cell suspension cultures of Catharanthus roseus.

Authors:  U Eilert; V Deluca; W G Kurz; F Constabel
Journal:  Plant Cell Rep       Date:  1987-07       Impact factor: 4.570

9.  GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.

Authors:  R A Jefferson; T A Kavanagh; M W Bevan
Journal:  EMBO J       Date:  1987-12-20       Impact factor: 11.598

10.  The juice of fresh leaves of Catharanthus roseus Linn. reduces blood glucose in normal and alloxan diabetic rabbits.

Authors:  Srinivas Nammi; Murthy K Boini; Srinivas D Lodagala; Ravindra Babu S Behara
Journal:  BMC Complement Altern Med       Date:  2003-09-02       Impact factor: 3.659
View more
  3 in total

1.  Genetic engineering approach using early Vinca alkaloid biosynthesis genes led to increased tryptamine and terpenoid indole alkaloids biosynthesis in differentiating cultures of Catharanthus roseus.

Authors:  Abhishek Sharma; Priyanka Verma; Archana Mathur; Ajay Kumar Mathur
Journal:  Protoplasma       Date:  2017-08-14       Impact factor: 3.356

2.  Development of efficient Catharanthus roseus regeneration and transformation system using agrobacterium tumefaciens and hypocotyls as explants.

Authors:  Quan Wang; Shihai Xing; Qifang Pan; Fang Yuan; Jingya Zhao; Yuesheng Tian; Yu Chen; Guofeng Wang; Kexuan Tang
Journal:  BMC Biotechnol       Date:  2012-06-29       Impact factor: 2.563

3.  Optimization of Agrobacterium mediated genetic transformation of cotyledonary node explants of Vigna radiata.

Authors:  Sushil Kumar Yadav; Sweety Katikala; Varalaxmi Yellisetty; Annapurna Kannepalle; Jyothi Lakshmi Narayana; Vanaja Maddi; Maheswari Mandapaka; Arun Kumar Shanker; Venkateswarlu Bandi; Kirti Pulugurtha Bharadwaja
Journal:  Springerplus       Date:  2012-12-10
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.