Literature DB >> 23571551

Analysis of Staphylococcal cassette chromosome mec in BD GeneOhm MRSA assay-negative strains.

Meng Zhang1, Teruyo Ito, Shanshuang Li, Shigeki Misawa, Shigemi Kondo, Takashi Miida, Akimichi Ohsaka, Keiichi Hiramatsu.   

Abstract

The BD GeneOhm MRSA assay could identify methicillin-resistant Staphylococcus aureus (MRSA) strains at a high ratio (97.8%). Analysis of 11 assay-negative MRSA strains suggested that insertion of non-mec staphylococcal cassette chromosome elements (SCCs) downstream of orfX, and carriage of SCCmecs with a left extremity that cannot be detected by the kit, might lead to their being given an incorrect negative status.

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Year:  2013        PMID: 23571551      PMCID: PMC3716159          DOI: 10.1128/AAC.00174-13

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN: 0066-4804            Impact factor:   5.191


  8 in total

1.  Performance of the BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) PCR assay for detecting MRSA nasal colonization in Taiwanese adults.

Authors:  Wei-Ting Chen; Jann-Tay Wang; Wen-Sen Lee; Cheng-Hua Huang; Chun-Hsing Liao; Yee-Chun Chen; Shan-Chwen Chang
Journal:  J Microbiol Immunol Infect       Date:  2010-10       Impact factor: 4.399

2.  Reliability of the BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) assay in detecting MRSA isolates with a variety of genotypes from the United States and Taiwan.

Authors:  Susan Boyle-Vavra; Robert S Daum
Journal:  J Clin Microbiol       Date:  2010-09-29       Impact factor: 5.948

3.  Comparison of BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) PCR versus the CHROMagar MRSA assay for screening patients for the presence of MRSA strains.

Authors:  John M Boyce; Nancy L Havill
Journal:  J Clin Microbiol       Date:  2007-11-21       Impact factor: 5.948

4.  Rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) in diverse clinical specimens by the BD GeneOhm MRSA assay and comparison with culture.

Authors:  Katja Lucke; Michael Hombach; Melanie Hug; Gaby E Pfyffer
Journal:  J Clin Microbiol       Date:  2010-01-13       Impact factor: 5.948

5.  Structural comparison of three types of staphylococcal cassette chromosome mec integrated in the chromosome in methicillin-resistant Staphylococcus aureus.

Authors:  T Ito; Y Katayama; K Asada; N Mori; K Tsutsumimoto; C Tiensasitorn; K Hiramatsu
Journal:  Antimicrob Agents Chemother       Date:  2001-05       Impact factor: 5.191

6.  Detection of methicillin-resistant Staphylococcus aureus (MRSA) in specimens from various body sites: performance characteristics of the BD GeneOhm MRSA assay, the Xpert MRSA assay, and broth-enriched culture in an area with a low prevalence of MRSA infections.

Authors:  Michael Hombach; Gaby E Pfyffer; Malgorzata Roos; Katja Lucke
Journal:  J Clin Microbiol       Date:  2010-09-22       Impact factor: 5.948

7.  High proportion of wrongly identified methicillin-resistant Staphylococcus aureus carriers by use of a rapid commercial PCR assay due to presence of staphylococcal cassette chromosome element lacking the mecA gene.

Authors:  Dominique S Blanc; Patrick Basset; Immaculée Nahimana-Tessemo; Katia Jaton; Gilbert Greub; Giorgio Zanetti
Journal:  J Clin Microbiol       Date:  2010-12-15       Impact factor: 5.948

8.  Comparison of the BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) PCR assay to culture by use of BBL CHROMagar MRSA for detection of MRSA in nasal surveillance cultures from an at-risk community population.

Authors:  Jason E Farley; Paul D Stamper; Tracy Ross; Mian Cai; Sharon Speser; Karen C Carroll
Journal:  J Clin Microbiol       Date:  2007-12-05       Impact factor: 5.948

  8 in total
  6 in total

1.  High frequency of occupied attB regions in Norwegian Staphylococcus aureus isolates supports a two-step MRSA screening algorithm.

Authors:  H S Tunsjø; S Kalyanasundaram; M M Worren; T M Leegaard; A E F Moen
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2016-09-09       Impact factor: 3.267

2.  Evaluation of the BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) assay as a method for detection of MRSA isolates, using a large collection of European and North African isolates.

Authors:  Sophie Trouillet-Assant; Michele Bes; Helene Meugnier; Sylvestre Tigaud; Jérôme Etienne; François Vandenesch; Frédéric Laurent
Journal:  J Clin Microbiol       Date:  2014-10-01       Impact factor: 5.948

3.  Transduction of staphylococcal cassette chromosome mec elements between strains of Staphylococcus aureus.

Authors:  Caitlyn R Scharn; Fred C Tenover; Richard V Goering
Journal:  Antimicrob Agents Chemother       Date:  2013-08-12       Impact factor: 5.191

4.  Introduction of a hydrolysis probe PCR assay for high-throughput screening of methicillin-resistant Staphylococcus aureus with the ability to include or exclude detection of Staphylococcus argenteus.

Authors:  Katja Bogestam; Martin Vondracek; Mattias Karlsson; Hong Fang; Christian G Giske
Journal:  PLoS One       Date:  2018-02-09       Impact factor: 3.240

5.  Updating Molecular Diagnostics for Detecting Methicillin-Susceptible and Methicillin-Resistant Staphylococcus aureus Isolates in Blood Culture Bottles.

Authors:  Fred C Tenover; Isabella A Tickler; Victoria M Le; Scott Dewell; Rodrigo E Mendes; Richard V Goering
Journal:  J Clin Microbiol       Date:  2019-10-23       Impact factor: 5.948

6.  Detection of Methicillin-Resistant Staphylococcus aureus Infections Using Molecular Methods.

Authors:  Fred C Tenover; Isabella A Tickler
Journal:  Antibiotics (Basel)       Date:  2022-02-12
  6 in total

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