Lodhi Santram1, A K Singhai. 1. Department of Pharmaceutical Sciences, Dr. H. S. Gour University SAGAR (M.P.), India.
Abstract
OBJECTIVE: To evaluate the wound healing potential of fractions from ethanol extract of Martynia annua (M. annua) Linn leaves. METHODS: Ethanol extract of M. annua Linn leaves was fractionate into three different fractions (MAF-A, MAF-B and MAF-C) which were screened for wound healing potential using two models: excision and incision on rats. The thin layer chromatography (TLC) profile of all fractions were analyzed and TLC of luteolin was also done. The Povidone-Iodine Ointment was used as reference for comparision. Excision and incision wounds were created on dorsal portion of rats for study. Wound contraction, biochemical parameters (protein level and hydroxyproline level) and histopathological study were performed in excision wound model whereas incision model was used for determination of tensile strength. RESULTS: The wound contraction and tensile strength of skin tissues were observed significantly greater in MAF-C fraction treated group than other two fractions (P<0.01). In excision wound method (on day 18) protein content and hydroxyproline were found significantly higher in MAF-C group than control group (P<0.01). Histopathological study also showed better angiogenesis, matured collagen fibres and fibroblast cells as compared with the control group. CONCLUSIONS: In conclusion, our findings suggest that fraction MAF-C from ethanol extract of M. annua leaves is found most effective in wound healing.
OBJECTIVE: To evaluate the wound healing potential of fractions from ethanol extract of Martynia annua (M. annua) Linn leaves. METHODS:Ethanol extract of M. annua Linn leaves was fractionate into three different fractions (MAF-A, MAF-B and MAF-C) which were screened for wound healing potential using two models: excision and incision on rats. The thin layer chromatography (TLC) profile of all fractions were analyzed and TLC of luteolin was also done. The Povidone-Iodine Ointment was used as reference for comparision. Excision and incision wounds were created on dorsal portion of rats for study. Wound contraction, biochemical parameters (protein level and hydroxyproline level) and histopathological study were performed in excision wound model whereas incision model was used for determination of tensile strength. RESULTS: The wound contraction and tensile strength of skin tissues were observed significantly greater in MAF-C fraction treated group than other two fractions (P<0.01). In excision wound method (on day 18) protein content and hydroxyproline were found significantly higher in MAF-C group than control group (P<0.01). Histopathological study also showed better angiogenesis, matured collagen fibres and fibroblast cells as compared with the control group. CONCLUSIONS: In conclusion, our findings suggest that fraction MAF-C from ethanol extract of M. annua leaves is found most effective in wound healing.
Authors: R J Nijveldt; E van Nood; D E van Hoorn; P G Boelens; K van Norren; P A van Leeuwen Journal: Am J Clin Nutr Date: 2001-10 Impact factor: 7.045
Authors: Muhammad Faraz Khan; Huaqiao Tang; James T Lyles; Rozenn Pineau; Zia-Ur-Rahman Mashwani; Cassandra L Quave Journal: Front Pharmacol Date: 2018-08-02 Impact factor: 5.810
Authors: Luiz Gonzaga do Nascimento-Neto; Romulo Farias Carneiro; Suzete Roberta Da Silva; Bruno Rocha Da Silva; Francisco Vassiliepe Sousa Arruda; Victor Alves Carneiro; Kyria Santiago Do Nascimento; Silvana Saker-Sampaio; Valdemiro Amaro Da Silva; Ana Lúcia Figueiredo Porto; Benildo Sousa Cavada; Alexandre Holanda Sampaio; Edson Holanda Teixeira; Celso Shiniti Nagano Journal: Mar Drugs Date: 2012-09-04 Impact factor: 6.085