OBJECTIVE: To study the effect of phage lysin on the growth of lysogens. METHODS: Sputum specimens processed by modified Petroff's method were respectively treated with phagebiotics in combination with lysin and lysin alone. The specimens were incubated at 37 °C for 4 days. At the end of day 1, 2, 3 and day 4, the specimens were streaked on blood agar plates and incubated at 37 °C for 18-24 hours. The growth of normal flora observed after day 1 was considered as lysogens. RESULTS: Sputum specimens treated with phagebiotics-lysin showed the growth of lysogens. When specimens treated with lysin alone, lysogen formation was avoided and normal flora was controlled. CONCLUSIONS: Lysin may have no effect on the growth of lysogens.
OBJECTIVE: To study the effect of phage lysin on the growth of lysogens. METHODS: Sputum specimens processed by modified Petroff's method were respectively treated with phagebiotics in combination with lysin and lysin alone. The specimens were incubated at 37 °C for 4 days. At the end of day 1, 2, 3 and day 4, the specimens were streaked on blood agar plates and incubated at 37 °C for 18-24 hours. The growth of normal flora observed after day 1 was considered as lysogens. RESULTS: Sputum specimens treated with phagebiotics-lysin showed the growth of lysogens. When specimens treated with lysin alone, lysogen formation was avoided and normal flora was controlled. CONCLUSIONS: Lysin may have no effect on the growth of lysogens.
Entities:
Keywords:
Bacteriophages; Normal flora; Phage lysin
Authors: Vanaja Kumar; S Balaji; N S Gomathi; P Venkatesan; Gomathi Sekar; K Jayasankar; P R Narayanan Journal: J Microbiol Methods Date: 2006-12-13 Impact factor: 2.363