OBJECTIVE: To compare the applicability of the SYBR Green-I assay with the standard schizont maturation assay, for determination of sensitivity of Plasmodium vivax (P. vivax) to chloroquine and a new antifolate WR 99210. METHODS: The study was conducted at Mae Tao Clinic for migrant workers, Tak Province during April 2009 to July 2010. A total of 64 blood samples (1 mL blood collected into sodium heparinized plastic tube) were collected from patients with mono-infection with P. vivax malaria prior to treatment with standard regimen of a 3-day chloroquine. In vitro sensitivity of P. vivax isolates was evaluated by schizont maturation inhibition and SYBR Green-I assays. RESULTS: A total of 30 out of 64 blood samples collected from patients with P. vivax malaria were successfully analyzed using both the microscopic schizont maturation inhibition and SYBR Green-I assays. The failure rates of the schizont maturation inhibition assay (50%) and the SYBR Green-I assay (54%) were similar (P=0.51). The median IC10s, IC50s and IC90s of both chloroquine and WR99210 were not significantly different from the clinical isolates of P. vivax tested. Based on the cut-off of 100 nM, the prevalences of chloroquine resistance determined by schizont maturation inhibition and SYBR Green-I assays were 19 and 11 isolates, respectively. The strength of agreement between the two methods was very poor for both chloroquine and WR99210. CONCLUSIONS: On the basis of this condition and its superior sensitivity, the microscopic method appears better than the SYBR Green-I Green assay for assessing in vitro sensitivity of fresh P. vivax isolates to antimalarial drugs.
OBJECTIVE: To compare the applicability of the SYBR Green-I assay with the standard schizont maturation assay, for determination of sensitivity of Plasmodium vivax (P. vivax) to chloroquine and a new antifolate WR 99210. METHODS: The study was conducted at Mae Tao Clinic for migrant workers, Tak Province during April 2009 to July 2010. A total of 64 blood samples (1 mL blood collected into sodium heparinized plastic tube) were collected from patients with mono-infection with P. vivaxmalaria prior to treatment with standard regimen of a 3-day chloroquine. In vitro sensitivity of P. vivax isolates was evaluated by schizont maturation inhibition and SYBR Green-I assays. RESULTS: A total of 30 out of 64 blood samples collected from patients with P. vivaxmalaria were successfully analyzed using both the microscopic schizont maturation inhibition and SYBR Green-I assays. The failure rates of the schizont maturation inhibition assay (50%) and the SYBR Green-I assay (54%) were similar (P=0.51). The median IC10s, IC50s and IC90s of both chloroquine and WR99210 were not significantly different from the clinical isolates of P. vivax tested. Based on the cut-off of 100 nM, the prevalences of chloroquine resistance determined by schizont maturation inhibition and SYBR Green-I assays were 19 and 11 isolates, respectively. The strength of agreement between the two methods was very poor for both chloroquine and WR99210. CONCLUSIONS: On the basis of this condition and its superior sensitivity, the microscopic method appears better than the SYBR Green-I Green assay for assessing in vitro sensitivity of fresh P. vivax isolates to antimalarial drugs.
Authors: Michele D Hastings; Jason D Maguire; Michael J Bangs; Peter A Zimmerman; John C Reeder; J Kevin Baird; Carol Hopkins Sibley Journal: Antimicrob Agents Chemother Date: 2005-02 Impact factor: 5.191
Authors: Varakorn Kosaisavee; Rossarin Suwanarusk; François Nosten; Dennis E Kyle; Marion Barrends; James Jones; Ric Price; Bruce Russell; Usa Lek-Uthai Journal: Exp Parasitol Date: 2006-03-20 Impact factor: 2.011
Authors: B Russell; F Chalfein; B Prasetyorini; E Kenangalem; K Piera; R Suwanarusk; A Brockman; P Prayoga; P Sugiarto; Q Cheng; E Tjitra; N M Anstey; R N Price Journal: Antimicrob Agents Chemother Date: 2008-01-07 Impact factor: 5.191
Authors: Lilia Gonzalez-Ceron; Mario H Rodriguez; Marco A Sandoval; Frida Santillan; Sonia Galindo-Virgen; Angel F Betanzos; Angel F Rosales; Olga L Palomeque Journal: Malar J Date: 2015-10-30 Impact factor: 2.979