Arun Kumar1. 1. Department of Biochemistry, International Medical School Management and Science University, MSU Holdings Sdn. Bhd.University Drive, Off Persiaran Olahraga, Section 13, 40100 Shah Alam, Selangor Darul Ehsan, Malaysia.
Abstract
OBJECTIVE: To investigate oxidative stress, hemoglobin percentage and erythrocyte osmotic fragility in various aging groups. METHODS: A total of 200 healthy volunteers of both genders between age group 20-65 years were selected by random method. Determination of hemoglobin percentage was done employing modified cyanide method of Dacie and Lewis. The erythrocyte lysis was observed in hypotonic solution of buffered saline at varying concentrations and optical density was measured at 540 nm. The extent of lipid peroxidation in form of malondialdehyde was measured by thiobarbituric acid method. RESULTS: The study found a significant decrease in hemoglobin percentage, increase in erythrocyte osmotic fragility and increased lipid peroxidation in form of malondialdehyde with increasing age. CONCLUSIONS: Supplementation of antioxidants may prevent the oxidative injury in elderly group of subjects.
OBJECTIVE: To investigate oxidative stress, hemoglobin percentage and erythrocyte osmotic fragility in various aging groups. METHODS: A total of 200 healthy volunteers of both genders between age group 20-65 years were selected by random method. Determination of hemoglobin percentage was done employing modified cyanide method of Dacie and Lewis. The erythrocyte lysis was observed in hypotonic solution of buffered saline at varying concentrations and optical density was measured at 540 nm. The extent of lipid peroxidation in form of malondialdehyde was measured by thiobarbituric acid method. RESULTS: The study found a significant decrease in hemoglobin percentage, increase in erythrocyte osmotic fragility and increased lipid peroxidation in form of malondialdehyde with increasing age. CONCLUSIONS: Supplementation of antioxidants may prevent the oxidative injury in elderly group of subjects.
Authors: Hae Young Chung; Matteo Cesari; Stephen Anton; Emanuele Marzetti; Silvia Giovannini; Arnold Young Seo; Christy Carter; Byung Pal Yu; Christiaan Leeuwenburgh Journal: Ageing Res Rev Date: 2008-07-18 Impact factor: 10.895
Authors: Sophia Sarpong-Kumankomah; Katherine B Knox; Michael E Kelly; Gary Hunter; Bogdan Popescu; Helen Nichol; Karen Kopciuk; Henry Ntanda; Jürgen Gailer Journal: PLoS One Date: 2022-01-12 Impact factor: 3.240