| Literature DB >> 23569252 |
Anjan K Saha1, Ferdinand Kappes, Amruta Mundade, Anja Deutzmann, David M Rosmarin, Maureen Legendre, Nicolas Chatain, Zeina Al-Obaidi, Barbara S Adams, Hidde L Ploegh, Elisa Ferrando-May, Nirit Mor-Vaknin, David M Markovitz.
Abstract
DEK is a biochemically distinct, conserved nonhistone protein that is vital to global heterochromatin integrity. In addition, DEK can be secreted and function as a chemotactic, proinflammatory factor. Here we show that exogenous DEK can penetrate cells, translocate to the nucleus, and there carry out its endogenous nuclear functions. Strikingly, adjacent cells can take up DEK secreted from synovial macrophages. DEK internalization is a heparan sulfate-dependent process, and cellular uptake of DEK into DEK knockdown cells corrects global heterochromatin depletion and DNA repair deficits, the phenotypic aberrations characteristic of these cells. These findings thus unify the extracellular and intracellular activities of DEK, and suggest that this paracrine loop involving DEK plays a role in chromatin biology.Entities:
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Year: 2013 PMID： 23569252 PMCID： PMC3637753 DOI： 10.1073/pnas.1220751110
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205