| Literature DB >> 23566843 |
Anthony Delalande1, Spiros Kotopoulis, Michiel Postema, Patrick Midoux, Chantal Pichon.
Abstract
Microbubbles first developed as ultrasound contrast agents have been used to assist ultrasound for cellular drug and gene delivery. Their oscillation behavior during ultrasound exposure leads to transient membrane permeability of surrounding cells, facilitating targeted local delivery. The increased cell uptake of extracellular compounds by ultrasound in the presence of microbubbles is attributed to a phenomenon called sonoporation. In this review, we summarize current state of the art concerning microbubble-cell interactions and cellular effects leading to sonoporation and its application for gene delivery. Optimization of sonoporation protocol and composition of microbubbles for gene delivery are discussed.Keywords: 1,2-dimyristoyl-sn-glycero-3-phosphocholine; 1,2-distearoyl-sn-glycero-3-phosphocholine; DMPC; DSPC; Gene therapy; HIFU; High Intensity Focused Ultrasound; Microbubbles; Physical gene delivery method; Ultrasound; VEGF; Vascular Endothelium Growth Factor; eGFP; enhanced Green Fluorescent Protein
Mesh：
Year: 2013 PMID： 23566843 DOI： 10.1016/j.gene.2013.03.095
Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688