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Literature DB >> 23563937

Rational design of a novel propeptide for improving active production of Streptomyces griseus trypsin in Pichia pastoris.

Zhenmin Ling¹, Yi Liu, Shaolei Teng, Zhen Kang, Jingjing Zhang, Jian Chen, Guocheng Du.

Abstract

Applying in silico simulations and in vitro experiments, the amino acid proline was proved to have a profound influence on Streptomyces griseus trypsinogen, and the hydrogen bond between H(57) and D(102) was found to be crucial for trypsin activity. By introducing an artificial propeptide, IVEF, the titer of trypsin was increased 6.71-fold.

Entities: Chemical Species

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Year: 2013 PMID： 23563937 PMCID： PMC3675915 DOI： 10.1128/AEM.00376-13

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

18 in total

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Journal: Appl Environ Microbiol Date: 2005-05 Impact factor: 4.792

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Authors: Zhenmin Ling; Tengbo Ma; Jianghua Li; Guocheng Du; Zhen Kang; Jian Chen
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10. Crystal structure analysis and refinement of two variants of trigonal trypsinogen: trigonal trypsin and PEG (polyethylene glycol) trypsinogen and their comparison with orthorhombic trypsin and trigonal trypsinogen.

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Journal: FEBS Lett Date: 1978-06-15 Impact factor: 4.124

4 in total

1. Improvement of catalytic efficiency and thermostability of recombinant Streptomyces griseus trypsin by introducing artificial peptide.

Authors: Zhenmin Ling; Zhen Kang; Yi Liu; Song Liu; Jian Chen; Guocheng Du
Journal: World J Microbiol Biotechnol Date: 2014-01-24 Impact factor: 3.312