Literature DB >> 23563551

Highly efficient transformation of the diatom Phaeodactylum tricornutum by multi-pulse electroporation.

Mado Miyahara1, Masaki Aoi, Natsuko Inoue-Kashino, Yasuhiro Kashino, Kentaro Ifuku.   

Abstract

A highly efficient nuclear transformation method was established for the pennate diatom Phaeodactylum tricornutum using an electroporation system that drives multi-sequence pulses to introduce foreign DNAs into the cells. By optimizing pulse conditions, the diatom cells can be transformed without removing rigid silica-based cell walls, and high transformation efficiency (about 4,500 per 10(8) cells) is achieved.

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Year:  2013        PMID： 23563551     DOI： 10.1271/bbb.120936

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN： 0916-8451            Impact factor:   2.043


  27 in total

Review 1.  Intracellular metabolic pathway distribution in diatoms and tools for genome-enabled experimental diatom research.

Authors:  Ansgar Gruber; Peter G Kroth
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2017-09-05       Impact factor: 6.237

Review 2.  Genetic and metabolic engineering in diatoms.

Authors:  Weichao Huang; Fayza Daboussi
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2017-09-05       Impact factor: 6.237

Review 3.  Genome editing in diatoms: achievements and goals.

Authors:  Peter G Kroth; Atle M Bones; Fayza Daboussi; Maria I Ferrante; Marianne Jaubert; Misha Kolot; Marianne Nymark; Carolina Río Bártulos; Andrés Ritter; Monia T Russo; Manuel Serif; Per Winge; Angela Falciatore
Journal:  Plant Cell Rep       Date:  2018-08-23       Impact factor: 4.570

4.  Establishment of Genetic Transformation in the Sexually Reproducing Diatoms Pseudo-nitzschia multistriata and Pseudo-nitzschia arenysensis and Inheritance of the Transgene.

Authors:  Valeria Sabatino; Monia Teresa Russo; Shrikant Patil; Giuliana d'Ippolito; Angelo Fontana; Maria Immacolata Ferrante
Journal:  Mar Biotechnol (NY)       Date:  2015-04-14       Impact factor: 3.619

5.  A stable and efficient nuclear transformation system for the diatom Chaetoceros gracilis.

Authors:  Kentaro Ifuku; Dongyi Yan; Mado Miyahara; Natsuko Inoue-Kashino; Yoshiharu Y Yamamoto; Yasuhiro Kashino
Journal:  Photosynth Res       Date:  2014-10-09       Impact factor: 3.573

6.  Droplet-based microfluidic screening and sorting of microalgal populations for strain engineering applications.

Authors:  Ziyi Yu; Katrin Geisler; Tina Leontidou; Rosanna E B Young; Sofie E Vonlanthen; Saul Purton; Chris Abell; Alison G Smith
Journal:  Algal Res       Date:  2021-06       Impact factor: 4.401

Review 7.  Diatom milking: a review and new approaches.

Authors:  Vandana Vinayak; Kalina M Manoylov; Hélène Gateau; Vincent Blanckaert; Josiane Hérault; Gaëlle Pencréac'h; Justine Marchand; Richard Gordon; Benoît Schoefs
Journal:  Mar Drugs       Date:  2015-04-29       Impact factor: 5.118

8.  Designer diatom episomes delivered by bacterial conjugation.

Authors:  Bogumil J Karas; Rachel E Diner; Stephane C Lefebvre; Jeff McQuaid; Alex P R Phillips; Chari M Noddings; John K Brunson; Ruben E Valas; Thomas J Deerinck; Jelena Jablanovic; Jeroen T F Gillard; Karen Beeri; Mark H Ellisman; John I Glass; Clyde A Hutchison; Hamilton O Smith; J Craig Venter; Andrew E Allen; Christopher L Dupont; Philip D Weyman
Journal:  Nat Commun       Date:  2015-04-21       Impact factor: 14.919

9.  Effect of an Introduced Phytoene Synthase Gene Expression on Carotenoid Biosynthesis in the Marine Diatom Phaeodactylum tricornutum.

Authors:  Takashi Kadono; Nozomu Kira; Kengo Suzuki; Osamu Iwata; Takeshi Ohama; Shigeru Okada; Tomohiro Nishimura; Mai Akakabe; Masashi Tsuda; Masao Adachi
Journal:  Mar Drugs       Date:  2015-08-20       Impact factor: 5.118

10.  Profiling of polar lipids in marine oleaginous diatom Fistulifera solaris JPCC DA0580: prediction of the potential mechanism for eicosapentaenoic acid-incorporation into triacylglycerol.

Authors:  Yue Liang; Yoshiaki Maeda; Tomoko Yoshino; Mitsufumi Matsumoto; Tsuyoshi Tanaka
Journal:  Mar Drugs       Date:  2014-05-28       Impact factor: 5.118
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