Literature DB >> 23559116

Ex vivo microbial leakage after using different final irrigation regimens with chlorhexidine.

Esther Navarro-Escobar1, Pilar Baca, María Paloma González-Rodríguez, María Teresa Arias-Moliz, Matilde Ruiz, Carmen María Ferrer-Luque.   

Abstract

OBJECTIVE: To assess the influence of final irrigation protocols with chlorhexidine in the coronal leakage of Enterococcus faecalis in filled root canals.
MATERIAL AND METHODS: Seventy single-root canals from extracted teeth were prepared using ProTaper instruments. The irrigation protocol accomplished an alternating irrigation with 5 mL of 2.5% sodium hypochlorite (NaOCI) and 17% EDTA between each file. The teeth were randomly divided into four experimental groups (n=15) according to the final irrigation regimen: group 1, without final irrigation; group 2, irrigation with 10 mL 2.0% chlorhexidine (CHX); group 3, with a final application of EC40™; and group 4, irrigation with the combination (1:1) of 0.2% CHX + 0.1% cetrimide (CTR). All the teeth were mounted in a two-chamber apparatus and the coronal access was exposed to E. faecalis. The presence of turbidity in the BHI broth over a period of 180 days was observed. The Friedman test was used for statistical analysis.
RESULTS: EC40™ varnish showed the least leakage at 180 days, and was statistically similar to 2% CHX. No significant differences were observed between the group without final irrigation and the 2% CHX group or 0.2% CHX + 0.1% CTR.
CONCLUSIONS: In this ex vivo study, EC40™ showed the longest delayed coronal leakage of E. faecalis, although without significant differences from 2% CHX.

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Year:  2013        PMID: 23559116      PMCID: PMC3881817          DOI: 10.1590/1678-7757201302322

Source DB:  PubMed          Journal:  J Appl Oral Sci        ISSN: 1678-7757            Impact factor:   2.698


  40 in total

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Review 2.  Root canal irrigants.

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3.  The effect of the canal-filled area on the bacterial leakage of oval-shaped canals.

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5.  Residual antimicrobial activity after canal irrigation with chlorhexidine.

Authors:  R R White; G L Hays; L R Janer
Journal:  J Endod       Date:  1997-04       Impact factor: 4.171

6.  Penetration of varnishes into demineralized root dentine in vitro.

Authors:  J Arends; H Duschner; J L Ruben
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7.  Influence of irrigation regimens on the adherence of Enterococcus faecalis to root canal dentin.

Authors:  Anil Kishen; Chee-Peng Sum; Shibi Mathew; Chwee-Teck Lim
Journal:  J Endod       Date:  2008-07       Impact factor: 4.171

8.  Residual antibacterial activity of chlorhexidine and MTAD in human root dentin in vitro.

Authors:  Zahed Mohammadi; Shahriar Shahriari
Journal:  J Oral Sci       Date:  2008-03       Impact factor: 1.556

9.  Antimicrobial action of calcium hydroxide, chlorhexidine and their combination on endodontic pathogens.

Authors:  V Ballal; M Kundabala; S Acharya; M Ballal
Journal:  Aust Dent J       Date:  2007-06       Impact factor: 2.291

10.  Evaluation of time required for recontamination of coronally sealed canals medicated with calcium hydroxide and chlorhexidine.

Authors:  B P F A Gomes; E Sato; C C R Ferraz; F B Teixeira; A A Zaia; F J Souza-Filho
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  2 in total

Review 1.  In vitro and ex vivo microbial leakage assessment in endodontics: A literature review.

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Journal:  J Int Soc Prev Community Dent       Date:  2016 Nov-Dec

2.  Solubility and bacterial sealing ability of MTA and root-end filling materials.

Authors:  Camila Galletti Espir; Juliane Maria Guerreiro-Tanomaru; Rubens Spin-Neto; Gisselle Moraima Chávez-Andrade; Fabio Luiz Camargo Villela Berbert; Mario Tanomaru-Filho
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