Literature DB >> 23554189

Fifty percent tissue culture infective dose assay for determining the titer of infectious human herpesvirus 8.

Sagar V Nadgir1, Heather R Hensler, Emilee R Knowlton, Charles R Rinaldo, Giovanna Rappocciolo, Frank J Jenkins.   

Abstract

We have developed a human herpesvirus 8 (HHV-8) 50% tissue culture infective dose (TCID50) assay using the T1H6-DC-SIGN cell line. Infection of T1H6-DC-SIGN cells with HHV-8 induces expression of β-galactosidase, which was used to determine TCID50 levels. Validation of TCID50 values was performed by immunofluorescence assay of HHV-8 infection of immature dendritic cells at various TCID50 doses.

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Year:  2013        PMID： 23554189      PMCID： PMC3716109          DOI： 10.1128/JCM.00761-13

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  24 in total

1.  Human herpesvirus 8 in semen and prostate.

Authors:  D J Blackbourn; J A Levy
Journal:  AIDS       Date:  1997-02       Impact factor: 4.177

2.  Infectious human herpesvirus 8 in a healthy North American blood donor.

Authors:  D J Blackbourn; J Ambroziak; E Lennette; M Adams; B Ramachandran; J A Levy
Journal:  Lancet       Date:  1997-03-01       Impact factor: 79.321

3.  Characterization of human herpesvirus 8 ORF59 protein (PF-8) and mapping of the processivity and viral DNA polymerase-interacting domains.

Authors:  S R Chan; B Chandran
Journal:  J Virol       Date:  2000-12       Impact factor: 5.103

4.  The KSHV viral interleukin-6 is not essential for latency or lytic replication in BJAB cells.

Authors:  Lei Chen; Michael Lagunoff
Journal:  Virology       Date:  2006-10-30       Impact factor: 3.616

5.  Identification of herpesvirus-like DNA sequences in AIDS-associated Kaposi's sarcoma.

Authors:  Y Chang; E Cesarman; M S Pessin; F Lee; J Culpepper; D M Knowles; P S Moore
Journal:  Science       Date:  1994-12-16       Impact factor: 47.728

6.  Transmission of human herpesvirus 8 infection from renal-transplant donors to recipients.

Authors:  N Regamey; M Tamm; M Wernli; A Witschi; G Thiel; G Cathomas; P Erb
Journal:  N Engl J Med       Date:  1998-11-05       Impact factor: 91.245

7.  Characterization of entry mechanisms of human herpesvirus 8 by using an Rta-dependent reporter cell line.

Authors:  Naoki Inoue; Jörn Winter; Renu B Lal; Margaret K Offermann; Shin Koyano
Journal:  J Virol       Date:  2003-07       Impact factor: 5.103

8.  Kaposi's sarcoma-associated herpesvirus-like DNA sequences in multicentric Castleman's disease.

Authors:  J Soulier; L Grollet; E Oksenhendler; P Cacoub; D Cazals-Hatem; P Babinet; M F d'Agay; J P Clauvel; M Raphael; L Degos
Journal:  Blood       Date:  1995-08-15       Impact factor: 22.113

9.  An immunotoxin targeting the gH glycoprotein of KSHV for selective killing of cells in the lytic phase of infection.

Authors:  Yingyun Cai; Edward A Berger
Journal:  Antiviral Res       Date:  2011-03-31       Impact factor: 10.103

10.  Traditional behavioural practices, the exchange of saliva and HHV-8 transmission in sub-Saharan African populations.

Authors:  J M Wojcicki
Journal:  Br J Cancer       Date:  2003-11-17       Impact factor: 7.640
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  7 in total

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2.  Cytopathic Effect Assay and Plaque Assay to Evaluate in vitro Activity of Antiviral Compounds Against Human Coronaviruses 229E, OC43, and NL63.

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3.  Proteomic Investigation Reveals Eukaryotic Translation Initiation Factor 5A Involvement in Porcine Reproductive and Respiratory Syndrome Virus Infection in vitro.

Authors:  Huawei Li; Bo Wan; Dawei Jiang; Pengchao Ji; Mengmeng Zhao; Xinfeng Li; Rui Li; Songlin Qiao
Journal:  Front Vet Sci       Date:  2022-04-13

4.  Human herpesvirus 8 induces polyfunctional B lymphocytes that drive Kaposi's sarcoma.

Authors:  Emilee R Knowlton; Giovanna Rappocciolo; Paolo Piazza; Lauren M Lepone; Sagar V Nadgir; Arlene Bullotta; Stella J Berendam; Jun Li; Todd A Reinhart; Frank J Jenkins; Charles R Rinaldo
Journal:  mBio       Date:  2014-09-02       Impact factor: 7.867

5.  Antiviral activity of mink interferon alpha expressed in the yeast Pichia pastoris.

Authors:  Hailing Zhang; Dongliang Zhang; Han Lu; Deying Zou; Bo Hu; Shizhen Lian; Shiying Lu
Journal:  Front Vet Sci       Date:  2022-09-14

6.  Human Herpesvirus 8 Infects and Replicates in Langerhans Cells and Interstitial Dermal Dendritic Cells and Impairs Their Function.

Authors:  Giovanna Rappocciolo; Mariel Jais; Paolo A Piazza; Diana C DeLucia; Frank J Jenkins; Charles R Rinaldo
Journal:  J Virol       Date:  2017-09-27       Impact factor: 5.103

7.  Multiplex PCR-based titration (MPBT) assay for determination of infectious titers of the three Sabin strains of live poliovirus vaccine.

Authors:  Hasmik Manukyan; Elvira Rodionova; Tatiana Zagorodnyaya; Tsai-Lien Lin; Konstantin Chumakov; Majid Laassri
Journal:  Virol J       Date:  2019-10-28       Impact factor: 4.099
  7 in total

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