PURPOSE: Solid tumors involve an inflammatory microenvironment portrayed by immune cells playing role in cancer progression via inflammatory p38α mitogen-activated protein kinase (MAPK) molecule that produces pro-inflammatory cytokines-TNFα, IL1β and IL6. This study quantified and compared the expression of p38α in peripheral blood mononuclear cells (PBMCs) of HNSCC patients with the healthy subjects. METHODS: The PBMC were isolated from the 35 control and 83 HNSCC patients. The expression of p38α in PBMCs was assessed using surface plasmon resonance (SPR), ELISA and western blot analysis. RESULTS: p38α levels were found to be over-expressed in HNSCC patients 0.98 ng/μl (95 % CI 0.95-1.02) as compared to controls 0.46 ng/μl (95 % CI 0.42-0.50) (p < 0.0001). CONCLUSION: p38α is over-expressed in PBMCs of HNSCC patients and may play a role in the progression of cancer. This research may translate a protein marker for HNSCC to clinical oncologist for therapeutic intervention and use as a predictive marker.
PURPOSE: Solid tumors involve an inflammatory microenvironment portrayed by immune cells playing role in cancer progression via inflammatory p38α mitogen-activated protein kinase (MAPK) molecule that produces pro-inflammatory cytokines-TNFα, IL1β and IL6. This study quantified and compared the expression of p38α in peripheral blood mononuclear cells (PBMCs) of HNSCC patients with the healthy subjects. METHODS: The PBMC were isolated from the 35 control and 83 HNSCC patients. The expression of p38α in PBMCs was assessed using surface plasmon resonance (SPR), ELISA and western blot analysis. RESULTS: p38α levels were found to be over-expressed in HNSCC patients 0.98 ng/μl (95 % CI 0.95-1.02) as compared to controls 0.46 ng/μl (95 % CI 0.42-0.50) (p < 0.0001). CONCLUSION: p38α is over-expressed in PBMCs of HNSCC patients and may play a role in the progression of cancer. This research may translate a protein marker for HNSCC to clinical oncologist for therapeutic intervention and use as a predictive marker.
Authors: L Katie Crosley; Susan J Duthie; Abigael C Polley; Freek G Bouwman; Carolin Heim; Francis Mulholland; Graham Horgan; Ian T Johnson; Edwin C Mariman; Ruan M Elliott; Hannelore Daniel; Baukje de Roos Journal: Genes Nutr Date: 2009-04-29 Impact factor: 5.523
Authors: Claudia A Dumitru; Maren K Fechner; Thomas K Hoffmann; Stephan Lang; Sven Brandau Journal: J Leukoc Biol Date: 2012-01-18 Impact factor: 4.962
Authors: Sokratis Trellakis; Kirsten Bruderek; Claudia A Dumitru; Hossein Gholaman; Xiang Gu; Agnes Bankfalvi; André Scherag; Jan Hütte; Nina Dominas; Götz F Lehnerdt; Thomas K Hoffmann; Stephan Lang; Sven Brandau Journal: Int J Cancer Date: 2011-03-29 Impact factor: 7.396