Literature DB >> 23551302

AMPK involvement in endoplasmic reticulum stress and autophagy modulation after fatty liver graft preservation: a role for melatonin and trimetazidine cocktail.

Mohamed Amine Zaouali1, Eleonora Boncompagni, Russel J Reiter, Mohamed Bejaoui, Isabel Freitas, Eirini Pantazi, Emma Folch-Puy, Hassen Ben Abdennebi, Francisco A Garcia-Gil, Joan Roselló-Catafau.   

Abstract

Ischemia/reperfusion injury (IRI) associated with liver transplantation plays an important role in the induction of graft injury. Prolonged cold storage remains a risk factor for liver graft outcome, especially when steatosis is present. Steatotic livers exhibit exacerbated endoplasmic reticulum (ER) stress that occurs in response to cold IRI. In addition, a defective liver autophagy correlates well with liver damage. Here, we evaluated the combined effect of melatonin and trimetazidine as additives to IGL-1 solution in the modulation of ER stress and autophagy in steatotic liver grafts through activation of AMPK. Steatotic livers were preserved for 24 hr (4°C) in UW or IGL-1 solutions with or without MEL + TMZ and subjected to 2-hr reperfusion (37°C). We assessed hepatic injury (ALT and AST) and function (bile production). We evaluated ER stress (GRP78, PERK, and CHOP) and autophagy (beclin-1, ATG7, LC3B, and P62). Steatotic livers preserved in IGL-1 + MEL + TMZ showed lower injury and better function as compared to those preserved in IGL-1 alone. IGL-1 + MEL + TMZ induced a significant decrease in GRP78, pPERK, and CHOP activation after reperfusion. This was consistent with a major activation of autophagic parameters (beclin-1, ATG7, and LC3B) and AMPK phosphorylation. The inhibition of AMPK induced an increase in ER stress and a significant reduction in autophagy. These data confirm the close relationship between AMPK activation and ER stress and autophagy after cold IRI. The addition of melatonin and TMZ to IGL-1 solution improved steatotic liver graft preservation through AMPK activation, which reduces ER stress and increases autophagy.
© 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Entities:  

Keywords:  AMPK; ER stress; IGL‐1 preservation solution; autophagy; cold ischemia/reperfusion injury; melatonin; steatotic liver; trimetazidine

Mesh:

Substances:

Year:  2013        PMID: 23551302     DOI: 10.1111/jpi.12051

Source DB:  PubMed          Journal:  J Pineal Res        ISSN: 0742-3098            Impact factor:   13.007


  42 in total

1.  ER stress: Autophagy induction, inhibition and selection.

Authors:  Harun-Or Rashid; Raj Kumar Yadav; Hyung-Ryong Kim; Han-Jung Chae
Journal:  Autophagy       Date:  2015-11-02       Impact factor: 16.016

Review 2.  Liver autophagy: much more than just taking out the trash.

Authors:  Jaime L Schneider; Ana Maria Cuervo
Journal:  Nat Rev Gastroenterol Hepatol       Date:  2013-11-05       Impact factor: 46.802

3.  Trimetazidine protects against myocardial ischemia/reperfusion injury by inhibiting excessive autophagy.

Authors:  Shiyong Wu; Guanglei Chang; Lei Gao; Dan Jiang; Liyou Wang; Guoxing Li; Xuexiu Luo; Shu Qin; Xueli Guo; Dongying Zhang
Journal:  J Mol Med (Berl)       Date:  2018-06-29       Impact factor: 4.599

Review 4.  Ischemic conditioning-induced endogenous brain protection: Applications pre-, per- or post-stroke.

Authors:  Yuechun Wang; Cesar Reis; Richard Applegate; Gary Stier; Robert Martin; John H Zhang
Journal:  Exp Neurol       Date:  2015-04-18       Impact factor: 5.330

Review 5.  Emerging concepts in liver graft preservation.

Authors:  Mohamed Bejaoui; Eirini Pantazi; Emma Folch-Puy; Pedro M Baptista; Agustín García-Gil; René Adam; Joan Roselló-Catafau
Journal:  World J Gastroenterol       Date:  2015-01-14       Impact factor: 5.742

6.  Effects of Institut Georges Lopez-1 and Celsior preservation solutions on liver graft injury.

Authors:  Donia Tabka; Mohamed Bejaoui; James Javellaud; Joan Roselló-Catafau; Jean-Michel Achard; Hassen Ben Abdennebi
Journal:  World J Gastroenterol       Date:  2015-04-14       Impact factor: 5.742

7.  GSK-3β mediates ischemia-reperfusion injury by regulating autophagy in DCD liver allografts.

Authors:  Tingting Liu; Yang Han; Wu Lv; Pan Qi; Wenshi Liu; Ying Cheng; Jianan Yu; Yongfeng Liu
Journal:  Int J Clin Exp Pathol       Date:  2019-02-01

8.  Technical feasibility of liver transplantation without cold storage.

Authors:  S Gül; F Klein; G Puhl; P Neuhaus
Journal:  Langenbecks Arch Surg       Date:  2013-12-08       Impact factor: 3.445

Review 9.  Snapshot: implications for melatonin in endoplasmic reticulum homeostasis.

Authors:  Wei Hu; Zhiqiang Ma; Shouyin Di; Shuai Jiang; Yue Li; Chongxi Fan; Yang Yang; Dongjin Wang
Journal:  Br J Pharmacol       Date:  2016-11-16       Impact factor: 8.739

10.  Ischemic preconditioning provides neuroprotection by induction of AMP-activated protein kinase-dependent autophagy in a rat model of ischemic stroke.

Authors:  Teng Jiang; Jin-Tai Yu; Xi-Chen Zhu; Qiao-Quan Zhang; Meng-Shan Tan; Lei Cao; Hui-Fu Wang; Jian-Quan Shi; Li Gao; Hao Qin; Ying-Dong Zhang; Lan Tan
Journal:  Mol Neurobiol       Date:  2014-05-10       Impact factor: 5.590

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