Literature DB >> 23545646

Overexpression, crystallization and preliminary X-ray crystallographic analysis of hypothetical protein SAV0479 from Staphylococcus aureus Mu50.

Chinar Pathak1, Sun Bok Jang, Hookang Im, Hye Jin Yoon, Bong Jin Lee.   

Abstract

SAV0479, a hypothetical protein from the Mu50 strain of methicillin- and vancomycin-resistant Staphylococcus aureus, was selected for structure and function determination as part of a structural genomics project. Here, the cloning, overexpression, purification and crystallization of SAV0479 are reported. Crystals were obtained by the hanging-drop vapour-diffusion method and diffraction data were collected to a resolution of 2.8 Å. The crystals belonged to space group P3(1)21, with unit-cell parameters a = b = 81.48, c = 82.53 Å. Three monomers of SAV0479 are present in each asymmetric unit.

Entities:  

Keywords:  hypothetical protein SAV0479; methicillin- and vancomycin-resistant Staphylococcus aureus

Mesh：

Substances：

Year:  2013        PMID： 23545646      PMCID： PMC3614165          DOI： 10.1107/S1744309113003783

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  18 in total

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Journal:  Clin Microbiol Rev       Date:  1997-10       Impact factor: 26.132

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Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2011-03-18
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